| 两种季铵盐阳离子表面活性剂对番茄灰霉病菌的毒理效应 |
| |
| 引用本文： | 李祥英,赵丽静,陈召亮,慕卫,刘峰.两种季铵盐阳离子表面活性剂对番茄灰霉病菌的毒理效应[J].植物保护学报,2011,38(3):265-270. |
| |
| 作者姓名： | 李祥英 赵丽静 陈召亮 慕卫 刘峰 |
| |
| 作者单位： | 1. 山东农业大学植物保护学院，山东省农药毒理与应用技术实验室，泰安271018
2. 山东农业大学植物保护学院，山东省农药毒理与应用技术实验室，泰安271018;上海农业科学院农产品保鲜加工研究中心，上海201106 |
| |
| 基金项目： | 公益性行业(农业)科研专项(200903033) |
| |
| 摘 要： | 为探讨季铵盐阳离子表面活性剂1227和C8-10对番茄灰霉病菌Botrytis cinerea的毒理效应,运用电子显微镜、氧电极、紫外-可见分光光度计等测定了1227、C8-10对番茄灰霉病菌分生孢子萌发、细胞壁结构、呼吸作用和三种细胞壁降解酶的影响,并在番茄叶片活体上接种经药剂处理的番茄灰霉病菌,观察药剂对其侵染活性的影响.结果显示,两种季铵盐阳离子表面活性剂5μg/mL处理可使分生孢子芽管变短变粗、基部明显膨大,显著抑制聚甲基半乳糖醛酸酶、多聚半乳糖醛酸酶和羧甲基纤维素酶的活性,并可显著影响番茄灰霉病菌在番茄活体叶片上的侵染活性；40μg/mL处理可导致菌体细胞壁表面出现多处破损、细胞内含物聚集等现象；100μg/mL处理显著抑制分生孢子的呼吸作用；250μg/mL和1 000μg/mL处理对病原菌菌丝呼吸产生破坏作用.说明两种季铵盐杀菌剂高剂量能够破坏菌体细胞壁结构,导致番茄灰霉病菌呼吸完全停止和死亡,而低剂量可以抑制细胞胞壁降解酶的活性,降低其致病力.
|
| 关 键 词： | 季铵盐阳离子表面活性剂 番茄灰霉病菌 毒理效应 |
| 收稿时间： | 8/1/2010 12:00:00 AM |
The toxicological effects of quaternary ammonium cationic surfactant to Botrytis cinerea |
| |
| Li Xiangying,Zhao Lijing,Chen Zhaoliang,Mu Wei and Liu Feng.The toxicological effects of quaternary ammonium cationic surfactant to Botrytis cinerea[J].Acta Phytophylacica Sinica,2011,38(3):265-270. |
| |
| Authors: | Li Xiangying Zhao Lijing Chen Zhaoliang Mu Wei and Liu Feng |
| |
| Institution: | College of Plant Protection, Shandong Agricultural University, Shandong Key Laboratory of Pesticide Toxicology and Application Technique, Tai'an 271018, Shandong Province, China;College of Plant Protection, Shandong Agricultural University, Shandong Key Laboratory of Pesticide Toxicology and Application Technique, Tai'an 271018, Shandong Province, China;College of Plant Protection, Shandong Agricultural University, Shandong Key Laboratory of Pesticide Toxicology and Application Technique, Tai'an 271018, Shandong Province, China; Agri-product Fresh Keeping and Processing Research Center, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China;College of Plant Protection, Shandong Agricultural University, Shandong Key Laboratory of Pesticide Toxicology and Application Technique, Tai'an 271018, Shandong Province, China;College of Plant Protection, Shandong Agricultural University, Shandong Key Laboratory of Pesticide Toxicology and Application Technique, Tai'an 271018, Shandong Province, China |
| |
| Abstract: | In order to study the toxicological effects of quaternary ammonium cationic surfactant to Botrytis cinerea,the toxicity morphology of conidia germination and cell wall were observed with microscope and scanning electronic microscope.The effect of quaternary ammonium cationic surfactant on the respiration of mycelial and conidia and the activity of cell wall degrading enzyme of B.cinerea were tested with the oxygen electrode method and UV-VIS spectrophotometry method.The activity of B.cinerea on tomato live ... |
| |
| Keywords: | quaternary ammonium cationic surfactants Botrytis cinerea toxicological effects |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《植物保护学报》浏览原始摘要信息 |
| 点击此处可从《植物保护学报》下载免费的PDF全文 |
|
