| 黄早四泛素连接酶Rbx1的分子克隆及原核表达 |
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| 引用本文： | 吴刘记,席章营,谢丽莉,陈彦惠.黄早四泛素连接酶Rbx1的分子克隆及原核表达[J].玉米科学,2011,19(3):43-46. |
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| 作者姓名： | 吴刘记 席章营 谢丽莉 陈彦惠 |
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| 作者单位： | 河南农业大学农学院,郑州,450002 |
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| 基金项目： | 国家“973”项目(2009CB118400)、河南省科技攻关计划项目(102102110171)、第47批中国博士后科学基金(20100470993) |
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| 摘 要： | 通过RT-PCR方法和基因克隆技术对黄早四Rbx1基因进行分子克隆和序列分析,构建黄早四Rbx1的原核表达系统,成功表达出带有His标签的黄早四Rbx1融合蛋白,并进一步利用His抗体,用蛋白免疫印迹的方法对重组蛋白进行鉴定。结果表明,重组蛋白能够与His抗体发生免疫反应,验证了黄早四Rbx1在原核表达系统中成功表达。
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| 关 键 词： | 玉米 黄早四 泛素连接酶Rbx1 分子克隆 原核表达 免疫印迹 |
| 收稿时间： | 6/8/2010 12:00:00 AM |
Molecular Cloning and Prokaryotic Expression of Ubiquitin Ligase Rbx1 from Huangzaosi |
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| WU Liu-ji,XI Zhang-ying,XIE Li-li,CHEN Yan-hui.Molecular Cloning and Prokaryotic Expression of Ubiquitin Ligase Rbx1 from Huangzaosi[J].Journal of Maize Sciences,2011,19(3):43-46. |
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| Authors: | WU Liu-ji XI Zhang-ying XIE Li-li CHEN Yan-hui |
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| Institution: | WU Liu-ji,XI Zhang-ying,XIE Li-li,CHEN Yan-hui(College of Agronomy,Henan Agricultural University,Zhengzhou 450002,China) |
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| Abstract: | Molecular cloning and sequence analysis of Huangzaosi Rbx1 were carried out based on RT-PCR method and gene cloning technique. At the same time, the prokaryotic expression system of Huangzaosi Rbx1 was constructed and the recombinant protein with a His-tag was successfully expressed. Furthermore, western blot with His antibody was used to identify the recombinant protein. The results showed that the recombinant protein had immune reaction with His antibody, and verified that Rbx1 from Huangzaosi could be successfully expressed in prokaryotic expression system. |
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| Keywords: | Maize Huangzaosi Ubiquitin Ligase Rbx1 Molecular cloning Prokaryotic expression Western blot |
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