利用稻草液体发酵产纤维素酶及酶粉提取的研究

利用摇瓶确定的优化培养基配方和产酶条件，在30 L罐中研究了里氏木霉HC -415菌利用稻草液体发酵产纤维素酶发酵液pH值、纤维素酶活性等随时间变化的动态规律，研究了发酵液纤维素酶的提取及得率等.所得未脱盐冻干纤维素酶粉CMC酶活性平均为355.0 IU/g， FPA平均为44.3 IU/g.相对发酵液得率平均为16.00 g/L.酶粉对发酵液CMC酶活性平均得率为77.16%， FPA酶活性平均得率为58.10%.

Cellulase Liquid Fermentation and Extraction with Straw

This experiment was carried out by Trichoderma reesei HC -415 according to Shake -flask orthogonal tests 1 and 2 and Shake -flask fermentation medium -2 test, which determined the consumption level of straw and bean cake powder in 30 L fermenter. We tracked the variation of pH and DO of fermentation liquor and the activity of cellulase changing over time, studied the extraction of cellulase, and calculated the yield. The average enzymic activity of the lyophilized cellulase of CMC was 355.0 IU/g, and 44.3 IU/g for FPA.The relative yield of fermentation liquor was 16.00 g/L.The enzymic activity yield of fermentation liquor for CMC was 77.16%,and 58.10% for FPA.