鸭肝炎病毒现场分离株单克隆抗体的研制

为有效防治鸭肝炎,建立特异性强、敏感性高、简便快速的DHV检测方法,对分离的DHV进行纯化,并制备单克隆抗体.鸭胚尿囊液中的DHV经冻融、氯仿反复处理、PEG浓缩和超速离心纯化后,免疫BALB/c小鼠,用杂交瘤技术,通过间接ELISA法筛选及多次亚克隆,成功获得8株阳性杂交瘤细胞,分别命名为DHV-1、DHV-6、DH...

Preparation and identification of monoclonal antibodies against the locale isolated strain of duck hepatitis virus

A kind of gesture segmentation method based on monocular vision was built for the analysis of the skin color information characteristics and gesture movements features, with which a meaningful gesture region could be obtained from the video image sequence. For the identified background image, the skin color region of gestures was captured through the analysis of the skin color information characteristics with a sampling interval of 10 frames. At the same time, the movement region of gestures was captured through the difference method, and then the initial gesture region could be obtained after the fusion of the skin color region and the movement region. The erosion and dilation algorithm of mathematical morphology were further studied, and the gestures segmentation and positioning of the video image sequence were realized. The experiment results have shown that the method has a good gesture segmentation results and higher positioning accuracy.