| 引起重庆主栽柑桔品种衰退病的病毒株系组群分析 |
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| 引用本文： | 王雪峰,周彦,刘科宏,唐科志,周常勇.引起重庆主栽柑桔品种衰退病的病毒株系组群分析[J].植物病理学报,2005,35(Z1):10-13. |
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| 作者姓名： | 王雪峰 周彦 刘科宏 唐科志 周常勇 |
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| 作者单位： | 1 中国农业科学院柑桔研究所, 重庆 400716;2 西南大学植物保护学院, 重庆 400712 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 柑桔衰退病毒(CTV)存在着许多生物学特性不同的株系。通过铲除感染强毒株植株或利用弱毒株交叉保护的方式来防治柑桔衰退病都需要对CTV株系进行准确、可靠的鉴定。本文根据对CTV衣壳蛋白基因(CPG)的限制性片段长度多态性(RFLP)分析,发现在重庆主栽柑桔品种的衰退病毒主要以CP/Hinf I RFLP第1、3和6组群为主,并且在田间以多株系混合感染为主。
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| 关 键 词： | 柑桔衰退病毒 限制性片段长度多态性 交叉保护 |
Analysis on CP/Hinf Ⅰ RFLP groups of Citrus tristeza virus in major citrus cultivars in Chongqing |
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| WANG Xue-feng,ZHOU Yan,LIU Ke-hong,TANG Ke-zhi,ZHOU Chang-yong.Analysis on CP/Hinf Ⅰ RFLP groups of Citrus tristeza virus in major citrus cultivars in Chongqing[J].Acta Phytopathologica Sinica,2005,35(Z1):10-13. |
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| Authors: | WANG Xue-feng ZHOU Yan LIU Ke-hong TANG Ke-zhi ZHOU Chang-yong |
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| Institution: | 1 Citrus Research Institute, Chinese Academy of Agricultural Science, Chongqing 400712, China;2 College of Plant Protection, Southwest University, Chongqing 400716, China |
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| Abstract: | Citrus tristeza virus (CTV) has a number of distinct strains differing in biological characteristics in citrus fields. Strategies such as eradication of severe strains-infected trees or using mild strains cross protection require accurate identification on virus strains. In the study, restriction fragment length polymorphism (RFLP) was used to examine the coat protein gene of CTV, and it was found that CP/Hinf I RFLP group 1, 3 and 6 were dominant in Chongqing. The results obtained by RFLP analysis revealed that mixture of CTV strains often occurred in field trees. |
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| Keywords: | CTV RFLP assay cross protection |
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