| 粮食污染物残留免疫分析技术研究与示范 |
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| 引用本文： | 张少波,张文生,李文辉,陈嘉东,许艇,郑妙,张小松,刘志平,肖治理,刘剑锋,万红艳,黄金萍,田巍,葛俊,赵娜. 粮食污染物残留免疫分析技术研究与示范[J]. 粮食储藏, 2017, 0(4): 5-14. DOI: 10.3969/j.issn.1000-6958.2017.04.002 |
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| 作者姓名： | 张少波 张文生 李文辉 陈嘉东 许艇 郑妙 张小松 刘志平 肖治理 刘剑锋 万红艳 黄金萍 田巍 葛俊 赵娜 |
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| 作者单位： | 1. 广东省粮食科学研究所 510050;2. 广州市粮食集团有限责任公司 510180;3. 中国农业大学 10094;4. 华南农业大学 510642;5. 深圳市粮食集团有限公司 518112;6. 广州市番禺粮食储备有限公司 511400 |
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| 基金项目： | 广州市民生科技研究(2014Y2-00520)，广东省科技项目(2014B070706001) |
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| 摘 要： | 随着人们对粮食安全问题的重视,政府部门不断加强对粮食安全的监测工作。针对粮食生产、加工、储运过程中的化学污染物安全性问题,研究粮食污染物快速监测技术,研究污染物免疫分析方法通过利用抗原、抗体的特异性反应来检测农药含量,设计并筛选吡虫啉、多溴联苯醚、功夫菊酯、氰戊菊酯4种半抗原,制备相关高特异性的抗体,分析吡虫啉、多溴联苯醚、功夫菊酯与气相色谱法(GC)相比,稻谷、大米、玉米样品通过相关性分析均高度相关,操作简便、快速。
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| 关 键 词： | 免疫分析法 多溴联苯醚 吡虫啉 噻虫嗪单克隆抗体 试剂盒 粮食安全 |
RESEARCH AND DEMONSTRATION OF IMMUNOASSAY TECHNOLOGY FOR CONTAMINENT OF GRAIN RESIDUES |
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| Zhang Shaobo,Zhang Wensheng,Li Wenhui,Chen Jiadong,Xu Ting,Zheng Miao,Zhang Xiaosong,Liu Zhiping,Xiao Zhili,Liu Jianfeng,Wan Hongyan,Huang Jinping,Tian Wei,Ge Jun,Zhao Na. RESEARCH AND DEMONSTRATION OF IMMUNOASSAY TECHNOLOGY FOR CONTAMINENT OF GRAIN RESIDUES[J]. www.ysfri.ac.cn, 2017, 0(4): 5-14. DOI: 10.3969/j.issn.1000-6958.2017.04.002 |
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| Authors: | Zhang Shaobo Zhang Wensheng Li Wenhui Chen Jiadong Xu Ting Zheng Miao Zhang Xiaosong Liu Zhiping Xiao Zhili Liu Jianfeng Wan Hongyan Huang Jinping Tian Wei Ge Jun Zhao Na |
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| Abstract: | With the people's attention to the issue of grain safety,government departments continue to strengthen the monitoring of grain safety.According to hazard problem of the chemical pollutants in grain production,processing,storage and transportation,fast grain pollutants monitoring technology was research,such as pollutant immunoassay by using specific reaction of antigen and antibody to detect pesticide content,design and screening of imidacloprid,PBDEs,cyhalothrin,fenvalerate 4 semi-antigen,preparation of high specificity antibody,analysis of imidacloprid,PBDEs,cyhalothrin.Compared with chromatography (GC),the method correlation analysis for paddy,rice,corn samples as highly correlated was simple and fast operation. |
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| Keywords: | immunoassay polybrominated diphenyl ethers imidacloprid imidacloprid monoclonal antibody ELISA kit grain safety |
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