Insecticide resistance status of the codling moth Cydia pomonella (Lepidoptera: Tortricidae) from Greece |
| |
Authors: | Costas Ch Voudouris Benoit Sauphanor Pierre Franck Maritza Reyes Zissis Mamuris John A Tsitsipis John Vontas John T Margaritopoulos |
| |
Institution: | aDepartment of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26, 41221 Larissa, Greece;bUR 1115, Plantes et Systèmes de culture Horticoles, INRA. Site Agroparc, 84914 Avignon Cedex 9, France;cInstituto de Producción y Sanidad Vegetal, Facultad de Ciencias Agrarias, Universidad Austral de Chile, Casilla 567, Valdivia, Chile;dLaboratory of Entomology and Agricultural Zoology, Department of Agriculture Crop Production and Rural Environment, University of Thessaly, Fytokou Str., 38446 Nea Ionia, Greece;eBiology Department, University of Crete, Vasilika Vouton, 71409 Heraklion, Crete, Greece |
| |
Abstract: | The codling moth Cydia pomonella L. is controlled mostly with chemical insecticides in Greece and control failures have been reported. However, there are no insecticide resistance studies in the country as yet. We examined the insecticide resistance status of 33 and 38 populations of fifth-instar non-diapausing and diapausing larvae, respectively by applying bioassays, biochemical and DNA diagnostics. Diagnostic concentrations of azinphos-methyl, phosalone, deltamethrin, thiacloprid, fenoxycarb, tebufenozide, methoxyfenozide and diflubenzuron were used in bioassays. Almost all populations showed reduced susceptibility to at least one insecticide and approximately half of them to all insecticides examined compared to a laboratory susceptible strain used as reference. However, only one out of six populations tested showed reduced susceptibility in ovicidal tests with fenoxycarb. Cross-resistances were observed among most insecticides, except from the pairs fenoxycarb–phosalone and thiacloprid–phosalone, in non-diapausing larvae. The more obvious biochemical marker associated with the reduced susceptibility observed in both larval instars was elevated cytochrome P450 polysubstrate monooxygenases activity, followed by elevated glutathione-S-transferase activity and reduced carboxylesterases activity. Neither sodium channel nor AChE known resistance mutations were found in any of the approximately 1000 individuals of each larval instar screened with diagnostic PCR. Actions for Integrated Resistance Management and application of alternative control methods are discussed. |
| |
Keywords: | Cydia pomonella Insecticides Resistance mechanisms Cross-resistance |
本文献已被 ScienceDirect 等数据库收录! |
|