Determination of genotoxicity of Fenaminosulf by Allium and Comet tests |
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Authors: | Recep Liman?brahim Hakk? Ci?erci Dilek Aky?lYasin Eren Muhsin Konuk |
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Institution: | Afyon Kocatepe University, Faculty of Science and Literatures, Biology Department, 03200 Afyonkarahisar, Turkey |
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Abstract: | Genotoxic effects of Fenaminosulf, fungicide and micro-biocide, were examined by using mitotic index (MI), mitotic phase, and Comet assay on the root meristem cells of Allium cepa. In the Allium root growth inhibition test, EC50 value was firstly determined as 25 ppm and, 12.5 (0.5 × EC50), 25 (EC50) and 50 (2 × EC50) ppm concentrations of Fenaminosulf were introduced to onion tuber roots. Distilled water was used as a negative control. All obtained data were subjected to statistical analyses by using SPSS 15.0 for Windows software. For comparison purposes, Duncan multiple range tests by using one-way analysis of variance (ANOVA) were employed and p < 0.05 was accepted as significant value. While MI (except in 24 h at 12.5 and 50 ppm) and prophase index increased, metaphase, anaphase and telophase indexes decreased in all concentrations compared to control at each exposure time. A significant increase in DNA damage was also observed at the concentration of 25 ppm in 24 h, 25 and 50 ppm in 96 h by Comet assay. |
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Keywords: | DNA damage Dexon Mitotic index Comet assay |
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