| 牛冠状病毒的分离鉴定 |
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| 引用本文： | 关新,朴范泽,侯喜林.牛冠状病毒的分离鉴定[J].黑龙江八一农垦大学学报,2007,19(2):55-58. |
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| 作者姓名： | 关新 朴范泽 侯喜林 |
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| 作者单位： | 黑龙江八一农垦大学动物科技学院,大庆,163319 |
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| 摘 要： | 将腹泻犊牛粪样上清接种于HCT-8细胞,出现CPE后收集病毒。提取病毒RNA后,巢氏反转录PCR扩增出冠状病毒N基因全长1437bp和N基因内部355bp片断,基因测序结果与Mebus株冠状病毒的同源性在98.3%,结果表明从腹泻的新生犊牛粪样中分离出的病毒确系牛冠状病毒。
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| 关 键 词： | 犊牛 冠状病毒 分离 鉴定 |
| 文章编号： | 1002-2090（2007）02-0055-04 |
| 收稿时间： | 2007-03-08 |
| 修稿时间： | 2007-03-08 |
Isolation and Identification of Bovine Coronavirus |
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| GUAN Xin,PIAO Fan-ze,HOU Xi-lin.Isolation and Identification of Bovine Coronavirus[J].Journal of Heilongjiang August First Land Reclamation University,2007,19(2):55-58. |
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| Authors: | GUAN Xin PIAO Fan-ze HOU Xi-lin |
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| Abstract: | The fecal suspension of a diarrheic calf was inoculated into HCT-8 cells,harvesting the virus when the CPE appeared. Virus RNA was extracted,and the Nested RT-PCR was performed to amplify the entire N genes with the length of 1437bp and a 355bp fragment of N gene in coronavirus. The homology comparison of the N genes sequence with Mebus strain was 98.3%. The result showed that a bovine coronavirus strain was isolated from diarrheic feces of a newborn calf. |
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| Keywords: | calf coronavirus isolation identification |
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