| SHR大鼠心室肌细胞分离方法改进及其L-型钙电流的观察 |
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| 引用本文： | 白旭,孙宏利,邢妍,刘宁. SHR大鼠心室肌细胞分离方法改进及其L-型钙电流的观察[J]. 东北农业大学学报, 2010, 41(3) |
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| 作者姓名： | 白旭 孙宏利 邢妍 刘宁 |
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| 作者单位： | 乳品科学教育部重点实验室,东北农业大学食品学院,哈尔滨,150030;哈尔滨医科大学大庆分校药理学教研室,黑龙江省大庆市哈尔滨医科大学大庆分校基础部,黑龙江,大庆,163319 |
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| 基金项目： | 黑龙江省科技攻关计划项目(GB01B407-02) |
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| 摘 要： | 文章探索建立简单可靠的SHR大鼠心室肌细胞的分离方法,提高细胞存活率和膜片钳试验成功率。采用Langendorff灌流装置,离体心脏逆向灌流,分离单个SHR大鼠心室肌细胞。使用膜片钳全细胞记录技术,记录大鼠心室肌细胞L-型钙电流。通过对细胞的观察发现,细胞呈长杆状,横纹清晰,细胞膜结构完整。膜片钳全细胞记录到典型的L-型钙电流,激活电位-40 mV,峰值电位0 mV,反转电位+50～+60 mV,呈现出电压依赖性。观察到的L-型钙电流可被钙通道阻滞剂维拉帕米抑制。结果表明,采用的大鼠心肌细胞分离方法可分离制备出适合膜片钳电生理观察的心肌细胞标本。
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| 关 键 词： | 心室肌细胞 膜片钳全细胞技术 L-型钙通道 维拉帕米 |
Improved method for isolation of SHR rats ventricular myocytes and observation of L-type calcium current |
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| BAI Xu,SUN Hongli,XING Yan,LIU Ning. Improved method for isolation of SHR rats ventricular myocytes and observation of L-type calcium current[J]. Journal of Northeast Agricultural University, 2010, 41(3) |
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| Authors: | BAI Xu SUN Hongli XING Yan LIU Ning |
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| Affiliation: | 1.Key Laboratory of Dairy Sciences;Ministry of Education;Food College;Northeast Agricultural University;Harbin 150030;China;2.Pharmacy Department;Daqing School;Harbin Medical University;Founda-tion Department;Daqing Heilongjiang 163319;China |
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| Abstract: | A simple and reliable SHR rat ventricular myocytes isolation method was explored and established to improve cell survival and the success rate of patch-clamp experiments.In present study,the isolation method of rats ventricular myocytes was conducted to investigate for the patch clamp experiment and observed the L-type calcium channel current.The Langendorff perfusion method was used to isolate the ventricular myocytes from SHR rats.The whole-cell patch clamp technique was used to record the calcium current... |
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| Keywords: | ventricular myocytes whole-cellpatch clamp L-type calcium channel Verapamil |
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