囊盘菌(Ascocoryne)挥发性气体成分多样性及其产烃分析

[目的]内生真菌紫胶囊盘菌(菌株NRRL 50072)首次被发现能产生石化燃料类似成分的挥发性有机代谢产物,这为生物质能源的发展开辟了新的途径,但囊盘菌属的真菌是否都具有该类代谢属性需要进一步明确。[方法]通过组织表面消毒分离囊盘菌不同菌株,经最大似然法构建分子系统发育树明确亲缘关系,利用顶空固相微萃取方法结合气质联用技术(HSSPEM-GC-MS),进行挥发性成分测定和分析。[结果]不同囊盘菌菌株均能产生挥发性烃、醇、酯、醛、酮和酸等代谢产物,但产物存在高度的多样性,即使近缘的菌株之间其挥发性产物也存在很大差异。[结论]囊盘菌属真菌可能普遍具有产挥发性烃类的能力,但其代谢产物种类具有菌株专化性。因此,发现或遗传工程创造目标新菌株资源是深入研究和开发利用囊盘菌真菌生物柴油的重要基础。

Metabolites Diversity and Hydrocarbons Analysis on Volatile Organic Compounds Produced by Ascocoryne Strains

[Objective] Endophytic fungus Ascocoryne sarcoides NRRL 50072 was firstly found to be able to produce volatile compounds similar to those in fossil fuels. This could be a new avenue to biofuel development. The purpose of this study is to clarify whether the other strains from the same species or related species in Ascocoryne have similar metabolisms to produce biofuel compounds. [Method] Different strains from Ascocoryne were isolated with tissue-surface sterilization. The evolutionary relationship between the strains in the genus was determined through constituting a ML phylogenetic tree. The compounds in volatile gas from the strains were analyzed and identified in NIST database with HSSPEM-GC-MS. [Result] All the strains studied were able to produce volatile metabolites containing hydrocarbons, alcohols, esters, aldehydes, acids, etc. However, the volatile compounds among the strains were highly diverse, even among closely related strains with significantly different compositions in their volatile compounds. [Conclusion] Most fungi from Ascocoryne could have the capability to produce volatile hydrocarbons. The compounds of hydrocarbons may be strain-specific. Therefore, it is fundamental for discovering or genetic engineering new strains to study and develop myco-diesel from Ascocoryne fungi.