Enzyme activities in a limed agricultural soil |
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Authors: | V Acosta-Martínez M A Tabatabai |
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Institution: | (1) Department of Agronomy, Iowa State University, Ames, IA 50011-1010, USA e-mail: malit@iastate.edu Fax: +1-515-2943163, US |
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Abstract: | This study assessed the effect of eight lime application rates, with four field replications, on the activities of 14 enzymes
involved in C, N, P, and S cycling in soils. The enzymes were assayed at their optimal pH values. The soil used was a Kenyon
loam located at the Northeast Research Center in Nashua, Iowa. Lime was applied in 1984 at rates ranging from 0 to 17,920 kg
effective calcium carbonate equivalent (ha–1), and surface samples (0–15 cm) were taken after 7 years. Results showed that organic C and N were not significantly affected
by lime application, whereas the soil pH was increased from 4.9 to 6.9. The activities of the following enzymes were assayed:
α- and β-glucosidases, α- and β-galactosidases, amidase, arylamidase, urease, l-glutaminase, l-asparaginase, l-aspartase, acid and alkaline phosphatases, phosphodiesterase, and arylsulfatase. With the exception of acid phosphatase,
which was significantly (P<0.001) but negatively correlated with soil pH (r=–0.69), the activities of all the other enzymes were significantly (P<0.001)and positively correlated with soil pH, with r values ranging from 0.53 for the activity of α-galactosidase to 0.89 for alkaline phosphatase and phosphodiesterase. The
Δ activity/Δ pH values ranged from 4.4 to 38.5 for the activities of the glycosidases, from 1.0 to 107 for amidohydrolases
and arylamidase, 97 for alkaline phosphatase, 39.4 for phosphodiesterase, and 11.2 for arylsulfatase. This value for acid
phosphatase was –35.0. The results support the view that soil pH is an important indicator of soil health and quality.
Received: 3 May 1999 |
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Keywords: | Soil quality Glycosidases Amidohydrolases Arylamidase Phosphatases |
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