|
|||||
|
|
| 黄心乌白菜游离小孢子培养及植株再生 | |
| 引用本文： | 汪维红,赵岫云,于拴仓,许 明,张凤兰,余阳俊,张德双.黄心乌白菜游离小孢子培养及植株再生[J].西北农业学报,2010,19(4):132-137. |
| 作者姓名： | 汪维红 赵岫云 于拴仓 许 明 张凤兰 余阳俊 张德双 |
| 作者单位： | 1. 沈阳农业大学,园艺学院,沈阳,110161;北京市农林科学院,蔬菜研究中心,北京,100097 2. 北京市农林科学院,蔬菜研究中心,北京,100097 3. 沈阳农业大学,园艺学院,沈阳,110161 |
| 基金项目： | 国家农业行业计划，现代农业产业技术体系建设专项资金资助，北京市科技计划项目 |
| 摘 要： | 为了提高黄心乌类型白菜的游离小孢子胚诱导率,选用7个淮南黄心乌品种,研究不同品种、取蕾月份和预处理对小孢子胚胎发生的影响,并采用流式细胞仪研究再生植株的倍性变异。结果表明,不同品种的小孢子出胚率存在明显差异,只有3个品种09-96、09-97和09-98诱导出了胚状体,平均出胚率分别为1.13胚/蕾,2.93胚/蕾和4.63胚/蕾;33℃热激处理对小孢子的胚发生至关重要;与对照相比,0.2%正丁醇预处理5 h显著提高了09-97和09-98的出胚率;倍性鉴定结果表明,在获得的再生植株中,单倍体、双单倍体、三倍体和四倍体都存在,但以双单倍体为主,09-97和09-98的双单倍体率分别达到70%和80%。 |
| 关 键 词： | 黄心乌 游离小孢子培养 丁醇预处理 |
Isolated Microspore Culture and Plantlet Regeneration in Huangxinwu Brassica rapa L ssp chinensis |
|
| WANG Weihong,ZHAO Xiuyun,YU Shuancang,XU Ming,ZHANG Fenglan,YU Yangjun and ZHANG Deshuang.Isolated Microspore Culture and Plantlet Regeneration in Huangxinwu Brassica rapa L ssp chinensis[J].Acta Agriculturae Boreali-occidentalis Sinica,2010,19(4):132-137. | |
| Authors: | WANG Weihong ZHAO Xiuyun YU Shuancang XU Ming ZHANG Fenglan YU Yangjun and ZHANG Deshuang |
| Institution: | WANG Weihong1,2,ZHAO Xiuyun2,YU Shuancang2,XU Ming1,ZHANG Fenglan2,YU Yangjun2 , ZHANG Deshuang2(1.College of Horticulture,Shenyang Agricultural University,Shenyang 110161,China,2.Beijing Academy of Agriculture , Forestry Science(BAAFS),Beijing 100097,China) |
| Abstract: | To improve the frequencies of microspore embrogenesis in Huangxinwu(Brassica rapa L ssp chinensis),seven varieties from Huainan area were employed to study the factors of enhancing the embrogenesis,including genotypes,culture season and pretreatment.In addition,the ploidy variation of regenerated plants were investigated by flow cytometry.The results showed that embrogenesis frequencies varied remarkably among varieties,and embryos were obtained only from three varieties,09-96(Huainantadiwu 1),09-97(Huainan... |
| Keywords: | Huangxinwu Isolated microspore culture n-butanol pretreatment |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《西北农业学报》浏览原始摘要信息 | |
| 点击此处可从《西北农业学报》下载免费的PDF全文 | |