| 鹅源新城疫病毒ZJ1株拯救体系相关因素的探索 |
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| 引用本文： | 张艳梅,提金凤,胡顺林,刘玉良,刘秀梵. 鹅源新城疫病毒ZJ1株拯救体系相关因素的探索[J]. 中国家禽, 2006, 28(13): 26-30 |
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| 作者姓名： | 张艳梅 提金凤 胡顺林 刘玉良 刘秀梵 |
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| 作者单位： | 扬州大学农业部畜禽传染病学重点开放实验室,江苏,扬州,225009 |
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| 基金项目： | 973项目(GI9990119)，国家自然科学基金重大项目(39893290) |
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| 摘 要： | 在构建了鹅源新城疫病毒(NewcastleDiseaseVirus,NDV)ZJ1毒株的微型基因组和表达该病毒NP、P与L蛋白的辅助质粒的基础上,通过对该病毒拯救体系中的重要影响因素如ZJ1在易感细胞的增殖、热休克对拯救效率的影响、表达T7RNA聚合酶的重组痘苗的感染复数、胞嘧啶阿拉伯呋喃糖苷(Arac)对重组痘苗复制的影响以及共转染质粒的比例和量等进行了探索。以上研究为该病毒的成功拯救及开展其它相关研究奠定了基础。
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| 关 键 词： | 新城疫病毒 病毒拯救体系 探索 |
| 收稿时间： | 2005-09-20 |
| 修稿时间： | 2005-09-20 |
Exploration of Rescue Condition for a Newcastle Disease Virus of Goose Origin |
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| Zhang Yanmei,Ti Jinfeng,Hu Shunlin,Liu Yuliang,Liu Xiufan. Exploration of Rescue Condition for a Newcastle Disease Virus of Goose Origin[J]. China Poultry, 2006, 28(13): 26-30 |
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| Authors: | Zhang Yanmei Ti Jinfeng Hu Shunlin Liu Yuliang Liu Xiufan |
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| Affiliation: | The Key Laboratory of Animal Infectious Diseases,Ministry of Agriculture, Yangzhou University,Yangzhou, JiangSu 225009 |
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| Abstract: | On the base of construction of the minigenome and the helper plasmids expressing NP,P and L proteins respectively of a Newcastle disease virus(NDV)of goose origin,exploration and comparison were done on the some important factors influencing NDV rescue system,such as the propagation of NDV ZJ1 strain on the sensitive cells,the effect of heat shock on the efficiency of rescue system,the multiplication of the recombinant vaccinia virus expressing T7 RNA polymerase,the effect of Arac on the replication of the recombinant vaccinia virus and the amount and proportion of the co-transfectant plasmids. The work mentioned above paved the way for successful rescue of NDV and other related research. |
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| Keywords: | NDV virus rescue exploration |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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