| 公农2号紫花苜蓿抗寒基因CAS19在烟草中的表达 |
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| 引用本文： | 帅丽芳,沈景林,郭瑞萍,唐鸿宇,丁雪梅,徐丽娟,刘莎莎,张梦晗,汤鑫.公农2号紫花苜蓿抗寒基因CAS19在烟草中的表达[J].安徽农业科学,2011,39(1):49-51. |
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| 作者姓名： | 帅丽芳 沈景林 郭瑞萍 唐鸿宇 丁雪梅 徐丽娟 刘莎莎 张梦晗 汤鑫 |
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| 作者单位： | 广州军区疾病控制中心;吉林大学畜牧兽医学院;山东畜牧兽医职业学院; |
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| 基金项目： | 国家高技术研究发展计划“863”项目(2008AA10Z224); 国家自然科学基金(30471229) |
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| 摘 要： | 目的]研究公农2号紫花苜蓿抗寒基因CAS19在烟草中的表达。方法]根据苜蓿抗寒基因（CAS19）核酸序列设计1对引物,用RT-PCR扩增出分离CAS19的蛋白基因,并克隆到pMD18-TVector载体中,再亚克隆到表达载体PBI121,成功构建重组表达质粒PB-CAS。经农杆菌介导转基因入烟草,并通过Sounthern-blotting杂交分析检测转基因苗。结果]CAS19抗寒基因可以在烟草中得以高效表达。结论]为进一步探明CAS19抗寒基因在烟草中的表达机制提供了理论依据。
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| 关 键 词： | CAS19 表达载体 烟草 紫花苜蓿 |
Expression of Cold Resistant Gene CAS19 of Gongnong No. 2 Medicago sativa L. in Tobacco |
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| SHUAI Li-fang et al.Expression of Cold Resistant Gene CAS19 of Gongnong No. 2 Medicago sativa L. in Tobacco[J].Journal of Anhui Agricultural Sciences,2011,39(1):49-51. |
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| Authors: | SHUAI Li-fang |
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| Institution: | SHUAI Li-fang et al (Center for Disease Control and Prevention Guangzhou Area,Guangzhou,Guangdong 510507) |
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| Abstract: | Objective] The aim was to study the expression of cold resistant gene CAS19 of Gongnong No.2 Medicago sativa L. in tobacco. Method] A pair of primers was designed according to nucleotide sequences of cold resistant gene CAS19 of M. sativa,and then RT-PCR was used to amplify the protein gene of CAS19,which was then cloned into pMD18-T vector and subcloned into expression vector PBI121. The recombination expression plasmid PBCAS was constructed. And then it was transferred into tobacco genome via Agrobacter... |
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| Keywords: | CAS19 Expression vector Tobacco Medicago sativa L |
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