| 小麦重组自交系群体高分子量麦谷蛋白亚基组成分析 |
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| 引用本文： | 李君,丁安明,崔法,王洪刚. 小麦重组自交系群体高分子量麦谷蛋白亚基组成分析[J]. 山东农业科学, 2011, 0(2): 11-14 |
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| 作者姓名： | 李君 丁安明 崔法 王洪刚 |
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| 作者单位： | 山东农业大学农学院/作物生物学国家重点实验室/国家小麦改良中心泰安分中心,山东,泰安,271018 |
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| 基金项目： | 国家“973”重大基础研究发展规划项目(2006CB101700)资助 |
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| 摘 要： | 以潍麦8号与济麦20为亲本构建的重组自交系群体RIL-8的468个系为材料,利用SDS-PAGE技术检测分析了其高分子量麦谷蛋白亚基（HMW-GS）及亚基组合构成特点。结果表明：供试RIL-8群体在Glu-A1、Glu-B1和Glu-D1三个位点共检测到12种等位变异,其中Glu-A1位点2种,Glu-B1位点5种,Glu-D1位点5种;共检测到23种HMW-GS组合,其中2种亲本类型,21种新类型;不同HMW-GS和亚基组合在群体中的分布频率存在较大差异。
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| 关 键 词： | 小麦 重组自交系 高分子量麦谷蛋白亚基 |
Analysis on HMW-GS Combination in Population of Recombinant Inbred Lines of Wheat |
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| LI Jun,DING An-ming,CUI Fa,WANG Hong-gang. Analysis on HMW-GS Combination in Population of Recombinant Inbred Lines of Wheat[J]. Shandong Agricultural Sciences, 2011, 0(2): 11-14 |
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| Authors: | LI Jun DING An-ming CUI Fa WANG Hong-gang |
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| Affiliation: | LI Jun,DING An-ming,CUI Fa,WANG Hong-gang(Agronomy College of Shandong Agricultural University/State Key Laboratory of Crop Biology/Taian Branch of National Wheat Improvement Center,Taian 271018,China) |
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| Abstract: | The high molecular weight glutenin subunits(HMW-GS) and their combinations in the F8 population of recombinant inbred lines were analyzed by sodium dodecyl sulphate polyacrylamide-gel electrophoresis(SDS-PAGE) consisting of 468 lines from the cross between the wheat cultivars Weimai 8 and Jimai 20.The results showed that 12 kinds of allelic variations of HMW-GS encoded by Glu-1 locus were detected,including 2,5 and 5 in Glu-A1,Glu-B1 and Glu-D1 respectively.Totally twenty-three combinations were detected,of... |
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| Keywords: | Wheat Recombinant inbred lines HMW-GS |
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