| 萝卜离体小孢子培养中的胚胎发生及发育途径 |
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| 引用本文： | 张丽,白小娟. 萝卜离体小孢子培养中的胚胎发生及发育途径[J]. 安徽农业科学, 2011, 39(1): 38-40 |
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| 作者姓名： | 张丽 白小娟 |
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| 作者单位： | 国家蔬菜工程技术研究中心,北京,100097;甘肃临夏州农业科学研究所,甘肃临夏,731100 |
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| 基金项目： | 国家科技部科技支撑计划项目“设施专用叶根菜类蔬菜高效育种技术研究与新品种选育”(2009BADB8B03) |
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| 摘 要： | [目的]观察萝卜离体小孢子培养中的胚胎发生及发育途径。[方法]以中国萝卜品种山东花叶心里美为试材,研究其离体小孢子培养中的细胞核分裂特征及发育途径。[结果]离体小孢子培养过程中出现2种细胞核分裂特征的差异：①进行配子体发育的不均等分裂,即分裂形成2个大小不等的营养核和生殖核,该类小孢子形态与正常发育中双核期的小孢子相同;②进行孢子体发育的均等分裂,即形成2个大小相等的营养核。对小孢子发育进一步进行观察发现,均等分裂的细胞继续发育形成四细胞结构、多细胞团、球形胚、心形胚、鱼雷形胚及子叶形胚。[结论]该研究结果为萝卜游离小孢子培养技术提供细胞学依据。
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| 关 键 词： | 萝卜 小孢子培养 发育途径 |
Embryogenesis and Development of Isolated Microspores of Raphanus sativus |
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| ZHANG Li et al. Embryogenesis and Development of Isolated Microspores of Raphanus sativus[J]. Journal of Anhui Agricultural Sciences, 2011, 39(1): 38-40 |
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| Authors: | ZHANG Li et al |
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| Affiliation: | ZHANG Li et al (National Engineering Research Center for Vegetables,Beijing 100097) |
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| Abstract: | [Objective]The aim was to observe embryogenesis and development of isolated microspores of Raphanus sativus. [Method] By using Chinese radish variety Shandong Huaye Xinlimei as the experimental materials,the caryocinesia features and development of isolated microspore in radish were studied. [Result] Two types of cell division appeared in the isolated microspore: unequal division and equational division.The unequal divided microspores had two different size nucleuses,while the equational divided microspores... |
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| Keywords: | Radish Microspore culture Embryogenesis |
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