| 黑刺李的组织培养与快速繁殖 |
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| 引用本文： | 吴建华,王立英,闵丽霞. 黑刺李的组织培养与快速繁殖[J]. 安徽农业科学, 2011, 39(1): 37+51-37,51 |
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| 作者姓名： | 吴建华 王立英 闵丽霞 |
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| 作者单位： | 种苗生物工程国家重点实验室;宁夏林业研究所股份有限公司; |
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| 基金项目： | 国家经济林木种苗快繁工程技术研究中心研究支持项目 |
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| 摘 要： | [目的]为黑刺李品种改良和工厂化繁育提供理论依据。[方法]以黑刺李幼嫩叶片为外植体,MS为基本培养基,分别对其进行启动、增殖及生根培养,建立黑刺李离体培养再生体系。[结果]外植体在启动培养基MS＋6-BA0.5mg/L＋NAA0.2mg/L＋2,4-D0.5mg/L上培养15d后边缘膨大,30d后形成愈伤组织,60d后形成丛生芽;带丛生芽的愈伤组织块在增殖培养基MS＋6-BA0.3mg/L＋NAA0.1mg/L上培养28d后陆续分化出3～5个丛芽;将增殖培养获得的2cm以上的无根小苗接种到生根培养基1/2MS＋IBA0.4mg/L＋蔗糖7.0g/L上培养15d后开始生根,25d后生根率达75%;将试管苗移栽到基质（草炭∶蛭石∶珍珠岩=1∶1∶2）上养护,30d后成活率达80%以上。[结论]该研究建立的黑刺李离体培养再生体系稳定性好、增殖速度快。
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| 关 键 词： | 黑刺李 离体培养 快速繁殖 愈伤组织 |
Tissue Culture and Rapid Propagation of Prunus spinosa |
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| WU Jian-hua et al. Tissue Culture and Rapid Propagation of Prunus spinosa[J]. Journal of Anhui Agricultural Sciences, 2011, 39(1): 37+51-37,51 |
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| Authors: | WU Jian-hua et al |
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| Affiliation: | WU Jian-hua et al (National Key Laboratory for Seedling Bioengineering,Yinchuan,Ningxia 750004) |
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| Abstract: | [Objective] The study aimed to provide theoretical basis for variety improvement and factory propagation of Prunus spinosa. [Method] With tender leaves of P. spinosa as explants,MS was used as basic medium for their starting,multiplication and rooting culture so as to establish its regeneration system for in vitro culture. [Result] After the explants were cultured on starting medium MS + 6-BA 0.5 mg/L + NAA 0.2 mg/L + 2,4-D 0.5 mg/L for 15 d,their edges were expanded,their calli were formed after 30 d and t... |
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| Keywords: | Prunus spinosa In vitro culture Rapid propagation Callus |
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