| 香菇EST-SSR标记的开发及应用 |
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| 引用本文： | 刘春滟,李南羿,张玉琼. 香菇EST-SSR标记的开发及应用[J]. 食用菌学报, 2010, 17(2) |
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| 作者姓名： | 刘春滟 李南羿 张玉琼 |
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| 作者单位： | 1. 安徽农业大学生命科学院,安徽合肥,230036
2. 浙江林学院农业与食品学院,浙江临安,311300 |
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| 基金项目： | 上海市农业遗传育种重点实验室开放课题 |
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| 摘 要： | 从NCBI中下载了12 184条香菇(Lentinula edodes)表达序列标签(expressed sequence tag,EST)序列,处理后得到全长3 681 177 bp的4 684个Unigene.在其中共发掘出142个简单重复序列(simple sequence repeat, SSR),分布于120条EST中,分布频率为2.99% (平均分布距离:25.924 kb).其中,单、二、三核苷酸重复是主要的重复类型,A/T,AG/CT,ACG/CTG是单、二、三核苷酸的主要重复基序,分别占所有EST-SSR的23.23%、21.83%和10.56%.利用引物设计软件PrimerPremier5.0设计了40对引物,并利用6%变性聚丙烯酰胺凝胶在18个香菇品种中进行检测.其中,22对引物为多态性引物,多态率为55%.应用NTSYS软件的聚类方法分析表明,18份材料遗传相似系数范围为0.375～0.984,与前人研究结果相符.
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| 关 键 词： | 香菇 EST序列 SSR标记 多态性 遗传多样性 |
Development of Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) Markers in Lentinula edodes |
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| LIU Chunyan,LI Nanyi,ZHANG Yuqiong. Development of Expressed Sequence Tag-Simple Sequence Repeat (EST-SSR) Markers in Lentinula edodes[J]. Acta Edulis Fungi, 2010, 17(2) |
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| Authors: | LIU Chunyan LI Nanyi ZHANG Yuqiong |
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| Affiliation: | LIU Chunyan1,LI Nanyi2,ZHANG Yuqiong11College of Life Science,Anhui Agricultural University,Hefei,Anhui 230036,China,2 College of Agriculture and Food,Zhejiang Forestry University,Lin'an,Zhejiang 311300 |
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| Abstract: | A total of 12 184 Lentinula edodes expressed sequence tags(ESTs) were downloaded from NCBI and shown to be associated with 4 684 Unigenes comprising 3 681 177 bp in length.A MISA search revealed a total of 142 simple sequence repeats(SSRs) with a frequency of 2.99% and an average distance distribution of 25.9 kb.The most abundant mononucleotide,dinuclotide and trinucleotide repeats were A/T,AG/CT and ACG/CTG,accounting for 23.23%,21.83% and 10.56% of each type,respectively.Forty EST-SSR primer pairs were de... |
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| Keywords: | Lentinula edodes expressed sequence tag(EST) simple sequence repeat(SSR) marker polymorphism genetic diversity |
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