| 柽柳硫氧还蛋白过氧化物酶(ThPrx1)基因转入酵母的抗逆能力分析 |
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| 引用本文： | 贺琳,蒋丽丽,王玉成. 柽柳硫氧还蛋白过氧化物酶(ThPrx1)基因转入酵母的抗逆能力分析[J]. 东北林业大学学报, 2011, 39(4): 101-104 |
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| 作者姓名： | 贺琳 蒋丽丽 王玉成 |
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| 作者单位： | 林木遗传育种与生物技术教育部重点实验室(东北林业大学); |
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| 基金项目： | 国家自然科学基金(30972387)资助 |
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| 摘 要： | 从柽柳(Tamarix hispida)中克隆到了一个硫氧还蛋白过氧化物酶(Peroxiredoxin,Prx)家族基因(ThPrx1).为了研究ThPrxl的抗逆功能,将ThPrx1基因构建到酵母表达载体pYES2中,转化到酿酒酵母(Saccharomyces cerevisiae)中,获得重组酵母,并用转空pYES...
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| 关 键 词： | 柽柳 硫氧还蛋白过氧化物酶 抗逆 酵母表达 |
Stress Tolerance of Thioredoxin Peroxidase Gene from Tamarix hispida(ThPrx1) Inserted into Yeast |
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| He Lin,Jiang Lili,Wang Yucheng. Stress Tolerance of Thioredoxin Peroxidase Gene from Tamarix hispida(ThPrx1) Inserted into Yeast[J]. Journal of Northeast Forestry University, 2011, 39(4): 101-104 |
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| Authors: | He Lin Jiang Lili Wang Yucheng |
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| Affiliation: | He Lin,Jiang Lili,Wang Yucheng(Key Laboratory of Forest Tree Genetic Improvement and Biotechnology(Northeast Forestry University),Ministry of Education,Harbin 150040,P.R.China) |
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| Abstract: | Full-length cDNA of Thioredoxin Peroxidase Gene(Prx1) was cloned from Tamarix hispida.To investigate the stress tolerance of PRX1 from T.hispida(ThPrx1),the recombinant plasmid designated as pYES2-ThPrx1 was constructed by inserting the ThPrx1 gene into the yeast expression vector pYES2.The plasmid pYES2-ThPrx1 was transformed into the yeast Saccharomyces cerevisiae,and the yeast transformed with empty pYES2 was used as the control.The recombinant yeast harboring ThPrx1 and the control yeast harboring empty... |
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| Keywords: | Tamarix hispida Thioredoxin peroxidase Stress tolerance Yeast expression |
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