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江西甘蔗花叶病病原的分子鉴定
引用本文:蒋军喜,谢艳,阙海勇. 江西甘蔗花叶病病原的分子鉴定[J]. 植物病理学报, 2009, 39(2): 203-206
作者姓名:蒋军喜  谢艳  阙海勇
作者单位:1 江西农业大学农学院, 南昌 330045;2 浙江大学生物技术研究所, 杭州 310029
摘    要: Sugarcane mosaic disease, caused by Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), Maize dwarf mosaic virus (MDMV) or Johnsongrass mosaic virus (JGMV) in Potyvirus, is one of the most important viral diseases of sugarcane. In the study, four primer pairs specific to SCMV, SrMV, MDMV and JGMV, respectively, were designed and used to detect 29 sugarcane leaf mosaic samples collected from 9 locations in Jiangxi province. The representative RT-PCR products were sequenced. The results showed that 22 samples were infected by SCMV, three by SrMV, and four were mix-infected by SCMV and SrMV. MDMV or JGMV were not identified in all samples. The result indicates that SCMV is the major pathogen of sugarcane mosaic disease in Jiangxi province, and SrMV is also a pathogen for the disease.

关 键 词:甘蔗花叶病毒  分子鉴定  mosaic  virus  马铃薯Y病毒属  玉米矮花叶病毒  病毒病害  病原
收稿时间:2008-01-10

Molecular identification of the pathogen of sugarcane mosaic disease in Jiangxi province
JIANG Junxi,XIE Yan,QUE Hai-yong. Molecular identification of the pathogen of sugarcane mosaic disease in Jiangxi province[J]. Acta Phytopathologica Sinica, 2009, 39(2): 203-206
Authors:JIANG Junxi  XIE Yan  QUE Hai-yong
Affiliation:1 College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China;2 Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China
Abstract:Sugarcane mosaic disease, caused by Sugarcane mosaic virus (SCMV), Sorghum mosaic virus (SrMV), Maize dwarf mosaic virus (MDMV) or Johnsongrass mosaic virus (JGMV) in Potyvirus, is one of the most important viral diseases of sugarcane. In the study, four primer pairs specific to SCMV, SrMV, MDMV and JGMV, respectively, were designed and used to detect 29 sugarcane leaf mosaic samples collected from 9 locations in Jiangxi province. The representative RT-PCR products were sequenced. The results showed that 22 samples were infected by SCMV, three by SrMV, and four were mix-infected by SCMV and SrMV. MDMV or JGMV were not identified in all samples. The result indicates that SCMV is the major pathogen of sugarcane mosaic disease in Jiangxi province, and SrMV is also a pathogen for the disease.
Keywords:sugarcane mosaic disease  SCMV  SrMV  molecular detection  
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