贵州乌头杀虫生物碱的研究

采用药典方法对贵州乌头Aconitum carmichaeli块根的粗总碱和纯化总碱进行了分析,粗总碱的质量分数为0.928%,纯化总碱的质量分数为0.683%,高于其他地区产乌头。通过萃取、硅胶柱层析、薄层制备和重结晶等方法从贵州乌头中分离得到3个主要生物碱,经熔点、电喷雾串联质谱(ESI-MS)及核磁共振等鉴定其分别为新乌头碱、次乌头碱和乌头碱。反相高压液相色谱分析显示,在贵州乌头中,新乌头碱、次乌头碱和乌头碱的质量分数分别为0.20%、0.10%和0.016%。

Insecticidal alkaloids from Aconitum carmichaeli produced in Guizhou

The crude and purified total alkaloid quantities of the Aconitum carmichaeli root collected in Guizhou Province were analyzed by the standard protocol in Chinese Pharmacopiea,which showed that the crude total alkaloid 0.928%,and the purified total alkaloid 0.683% are higher in quantity than A.carmichaeli produced in the other places.Three main alkaloids were isolated by solvent extract,silica gel chromatography,thin layer chromatography preparation,recrystallization,and they were identified as hypaconitine,mesaconitine and aconitine by m.p.,ESI-MS,¹H and ¹³C NMR spectral data.The reverse HPLC analysis indicated that the quantities of mesaconitine,hypaconitine,and aconitine in Guizhou Aconitum carmichaeli are 0.20%,0.10% and 0.016%,respectively.