Determination of 4,4'-dinitrocarbanilide (DNC), the active component of the antifertility agent nicarbazin, in chicken, duck, and goose plasma |
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| Authors: | Primus T M Kohler D J Goodall M A Yoder C Griffin D Miller L Johnston J J |
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| Institution: | Analytical Chemistry Project, U.S. Department of Agriculture/Animal and Plant Health Inspection Service/National Wildlife Research Center, 4101 LaPorte Avenue, Fort Collins, Denver, Colorado 80521, USA. thomas.m.primus@usda.gov |
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| Abstract: | 4,4'-Dinitrocarbanilide (DNC) was extracted from chicken, duck, and goose plasma and isolated by reversed-phase high-performance liquid chromatography. DNC was detected by ultraviolet absorbance at 347 nm and quantified by comparison to a calibration standard. Recovery data were determined by analyzing DNC-fortified control plasma. The mean recovery of DNC in fortified chicken plasma samples was 99.7 +/- 1.9% for 0.18 and 9.1 ppm DNC, and in fortified duck and goose plasma samples was 99.5 +/- 4.9% and 101.4 +/- 4.5%, respectively, for 0.18, 9.1, and 18 ppm DNC. |
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