| 蝎子草醇提浸膏对金黄色葡萄球菌抗菌机制的研究 |
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| 引用本文： | 江震献,彭霞,张晓林,朱玲,李海霞,陈章宝.蝎子草醇提浸膏对金黄色葡萄球菌抗菌机制的研究[J].西南农业大学学报,2011,33(5). |
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| 作者姓名： | 江震献 彭霞 张晓林 朱玲 李海霞 陈章宝 |
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| 作者单位： | 西南大学药学院;重庆市兽药工程技术研究中心; |
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| 基金项目： | 重庆市科委重点攻关资助项目(CSTC2009AB1181) |
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| 摘 要： | 目的:研究蝎子草醇提浸膏(Girardinia suborbiculata C.J.Chen ethanol extract,GSEE)对金黄色葡萄球菌的抗菌机制.方法:测定蝎子草醇提浸膏对金黄色葡萄球菌的最低抑菌浓度(MIC),通过GSEE对菌体生长曲线、菌体可溶性蛋白的表达和菌体核酸合成等的影响来对GSEE的抗菌机制进行研究.结果:GSEE对金黄色葡萄球菌的MIC为100 mg/mL,可明显抑制金黄色葡萄球菌对数生长期的菌体分裂;SDS-PAGE电泳表明,金黄色葡萄球菌的可溶性蛋白含量在GSEE作用后的条带明显少于对照组;同时,通过DAPI染色显示,GSEE对菌体的核酸合成具有显著抑制作用.结论:GSEE对金黄色葡萄球菌具有较强的抗菌作用,其抗菌机制可能通过抑制菌体可溶性蛋白的表达和菌体核酸的合成等方面来实现抑制金黄色葡萄球菌的生长.
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| 关 键 词： | 蝎子草 抗菌机制 抗菌活性 |
Studies on Antibacterial Mechanism of Ethanol Extract from Girardinia suborbiculata C.J.Chen on Staphylococcus aureus |
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| JIANG Zhen-xian,PENG Xia,ZHANG Xiao-lin,ZHU Ling,LI Hai-xia,CHEN Zhang-bao,.School of Pharmaceutical Sciences,Southwest University,Chongqing ,China,.Chongqing Engineering Technology Research Center of Zoopharmaceuticals.Studies on Antibacterial Mechanism of Ethanol Extract from Girardinia suborbiculata C.J.Chen on Staphylococcus aureus[J].Journal of Southwest Agricultural University,2011,33(5). |
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| Authors: | JIANG Zhen-xian PENG Xia ZHANG Xiao-lin ZHU Ling LI Hai-xia CHEN Zhang-bao School of Pharmaceutical Sciences Southwest University Chongqing China Chongqing Engineering Technology Research Center of Zoopharmaceuticals |
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| Institution: | JIANG Zhen-xian1,PENG Xia1,ZHANG Xiao-lin1,ZHU Ling1,LI Hai-xia1,CHEN Zhang-bao1,21.School of Pharmaceutical Sciences,Southwest University,Chongqing 400715,China,2.Chongqing Engineering Technology Research Center of Zoopharmaceuticals |
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| Abstract: | The minimal inhibitory concentration(MIC) of the ethanol extract from Girardinia suborbiculata C.J.Chen(GSEE) against Staphylococcus aureus was determined and the influences of GSEE on the growth curve,soluble proteins expression and cellular nucleic acid synthesis of the bacterium were detected so as to study the antibacterial mechanism of GSEE on S.aureus.The results showed that MIC of GSEE against S.aureus was 100 mg/mL.SDS-PAGE indicated that the content of cellular soluble proteins in GSEE treatment de... |
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| Keywords: | Girardinia suborbiculata C J Chen antibacterial mechanism antibacterial activity |
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