| 高效溶栓菌株的筛选鉴定及活性测定 |
| |
| 引用本文： | 霍金柱,陈斌,李莹,何正波,敬俊锋.高效溶栓菌株的筛选鉴定及活性测定[J].西南农业大学学报,2011,33(6). |
| |
| 作者姓名： | 霍金柱 陈斌 李莹 何正波 敬俊锋 |
| |
| 作者单位： | 重庆师范大学昆虫与分子生物学研究所;重庆高校生物活性物质工程研究中心;活性物质生物技术教育部工程研究中心; |
| |
| 基金项目： | 重庆市自然科学基金重点项目(No.CSTC2008BA5030) |
| |
| 摘 要： | 以日本纳豆、重庆江津豆豉、忠县豆腐乳为原料,采用酪蛋白平板初筛、摇瓶复筛,得到18株产溶栓酶的菌株.通过纤维蛋白平板实验,从忠县豆腐乳中筛选出1株具有较高溶栓活性的菌株FR-033(登录号:HQ009804),菌株经液体发酵后获得含酶发酵液,采用硫酸铵分段盐析及sephadexG-50凝胶层析进行纯化,获得较高纯度的溶栓酶,酶活力为2 163 IU/mL.进一步对FR-033菌株的形态和生理生化特征进行研究.利用细菌16S rDNA通用引物对16S rRNA进行PCR扩增并测序,得到1444 bp长度的DNA序列,通过BLAST软件在NCBI网站收索其同源性性序列,利用PAUP4.0和MAGE4软件绘制系统发育树,结果表明:菌株FR-033与枯草芽孢杆菌(Bacil-lus subtilis)的16S rRNA序列同源性达99%以上,在系统发育树中,菌株FR-033与枯草芽孢杆菌在同一支上,且遗传距离最短;FR-033的形态及大部分生理生化特征与枯草芽孢杆菌极为相似,表明该菌株是枯草芽孢杆菌(Ba-cillus subtilis)的一个高效的产溶栓酶菌株.
|
| 关 键 词： | 豆腐乳 枯草芽孢杆菌 活性测定 16S rRNA |
Screening and Determination of a Fibrinolytic Enzyme-Producing Strain of Bacillus subtilis and Its Activity Assay |
| |
| HUO Jin-zhu,CHEN Bin,LI Ying,HE Zheng-bo,GOU Jun-feng .Chongqing Key Laboratory of Animal Biology,Chongqing Normal University,Chongqing ,China,.Chongqing Engineering Research Center of Bioactive Substances.Screening and Determination of a Fibrinolytic Enzyme-Producing Strain of Bacillus subtilis and Its Activity Assay[J].Journal of Southwest Agricultural University,2011,33(6). |
| |
| Authors: | HUO Jin-zhu CHEN Bin LI Ying HE Zheng-bo GOU Jun-feng Chongqing Key Laboratory of Animal Biology Chongqing Normal University Chongqing China Chongqing Engineering Research Center of Bioactive Substances |
| |
| Institution: | HUO Jin-zhu,CHEN Bin,LI Ying,HE Zheng-bo,GOU Jun-feng 1.Chongqing Key Laboratory of Animal Biology,Chongqing Normal University,Chongqing 400047,China,2.Chongqing Engineering Research Center of Bioactive Substances,3.Institute of Entomology and Molecular Biology |
| |
| Abstract: | Bacillus natto can be separated from Japanese traditional fermented food and it has the function of dissolving thrombus.Whether similar strains can be separated and identified from Chinese traditional fermented foods needs further studies.In an experiment reported in this paper,imported Japanese natto,Jiangjin douche(a fermented soybean) and Zhongxian pickle bean curd were used as the raw materials.A total of 18 fibrinolytic enzyme-producing strains were obtained,using the method of casein flat as the initi... |
| |
| Keywords: | kpickle bean curd Bacillus subtilis activity determination 16S rRNA |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《西南农业大学学报》浏览原始摘要信息 |
| 点击此处可从《西南农业大学学报》下载免费的PDF全文 |
|
