| 规模化猪场猪繁殖与呼吸综合征感染混合细菌研究 |
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| 引用本文： | 周英顺,王红宁,杨鑫,邹立扣,张安云,曾博,管仲兵,徐昌文.规模化猪场猪繁殖与呼吸综合征感染混合细菌研究[J].西南农业大学学报,2011,33(4). |
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| 作者姓名： | 周英顺 王红宁 杨鑫 邹立扣 张安云 曾博 管仲兵 徐昌文 |
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| 作者单位： | 四川大学生命科学学院动物疫病防控与食品安全四川省重点实验室985工程西南资源环境与灾害防治科技创新平台; |
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| 基金项目： | 国家“十一五”科技支撑计划资助项目(2007BAD51B05); “荣昌猪健康养殖生物安全控制技术研究与示范”资助项目(2006BAD14B05-05) |
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| 摘 要： | 规模化猪场爆发急性高热病,采集死猪肺脏病料和血清5份用RT-PCR方法进行PRRSVNSP2基因扩增,3份为猪繁殖与呼吸综合征阳性,编号为scwhn01,scwhn02和scwhn03.对scwhn01 NSP2基因进行克隆和序列测定分析,有30个氨基酸的不连续缺失,与VR2332,JXA1,BJ-4,HuN4,CH-1a,HB-1(sh)2002和HB-2(sh)2002的NSP2的同源率在74.2%~91.0%之间,并从病料中分离到2株细菌,编号分别为SNC0901和SNC0902,均使被攻毒小鼠死亡.16S rDNA测序并在Genbank中blast鉴定为副猪嗜血杆菌和大肠杆菌.对所分离的细菌进行17种抗生素药敏实验,对头孢噻呋、头孢噻吩等敏感;对克林霉素、阿莫西林/棒酸等中度敏感;对荷兰、林可霉素、丁胺卡那霉素和土霉素等耐药.表明该猪场因高致病性蓝耳病病毒混合感染副猪嗜血杆菌、大肠杆菌导致猪大量死亡.
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| 关 键 词： | 猪繁殖与呼吸综合征 副猪嗜血杆菌 大肠杆菌 混合感染 |
Porcine Reproductive and Respiratory Syndrome and Mixed Bacteria Infection on a Large-Scale Pig Farm |
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| ZHOU Ying-shun,WANG Hong-ning,YANG Xin,ZHANG An-yun,ZOU Li-kou,ZENG Bo,GUAN Zhong-bin,XU Chang-wen School of Life Science,Sichuan University,Animal Disease Prevention , Food Safety Key Laboratory of Sichuan Province, Project Science Innovative Platform for Resource , Environment Protection of Southwestern China,Chengdu ,China.Porcine Reproductive and Respiratory Syndrome and Mixed Bacteria Infection on a Large-Scale Pig Farm[J].Journal of Southwest Agricultural University,2011,33(4). |
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| Authors: | ZHOU Ying-shun WANG Hong-ning YANG Xin ZHANG An-yun ZOU Li-kou ZENG Bo GUAN Zhong-bin XU Chang-wen School of Life Science Sichuan University Animal Disease Prevention Food Safety Key Laboratory of Sichuan Province Project Science Innovative Platform for Resource Environment Protection of Southwestern China Chengdu China |
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| Institution: | ZHOU Ying-shun,WANG Hong-ning,YANG Xin,ZHANG An-yun,ZOU Li-kou,ZENG Bo,GUAN Zhong-bin,XU Chang-wen School of Life Science,Sichuan University,Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province,985 Project Science Innovative Platform for Resource and Environment Protection of Southwestern China,Chengdu 610064,China |
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| Abstract: | Outbreak of an acute high fever disease occurred on a large-scale pig farm.Five samples of lung tissue and serum were collected from the death pig,and RT-PCR was performed to amplify the porcine reproductive and respiratory syndrome NSP2 gene.Three samples proved to be PRRSV-positive and were designated as scwhn01,scwhn02 and scwhn03.The Nsp2 genes of scwhn01 were cloned and sequenced.The results revealed discontinuous deletions of 90 nucleotide of the Nsp2 gene.Sequence analysis showed that the Nsp2 gene s... |
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| Keywords: | porcine reproductive and respiratory syndrome Haemophilus parasuis Escherichia coli mixed infection |
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