| qRealTime-PCR检测CsWIP1在柑橘不同组织中的表达 |
| |
| 引用本文： | 杨雪莲,闫树堂,贝学军,刘斌,魏召新.qRealTime-PCR检测CsWIP1在柑橘不同组织中的表达[J].西南农业大学学报,2011,33(4). |
| |
| 作者姓名： | 杨雪莲 闫树堂 贝学军 刘斌 魏召新 |
| |
| 作者单位： | 西南大学园艺园林学院;贵州大学农学院;中国农科院柑橘研究所; |
| |
| 基金项目： | 重庆市科技攻关计划项目“柑橘重要功能基因克隆与功能分析”(CSTC,2007AA1018); 贵州大学引进人才科研项目(贵大人基合字(2010)017号)资助 |
| |
| 摘 要： | 通过实时荧光定量PCR(qRealTime-PCR)检测柑橘伤害诱导基因(CsWIP1)在乙烯胁迫和机械伤害过程中的表达情况.结果显示:CsWIP1仅在外源乙烯处理的叶片和果实离层(AZ-C)中表达,而在伤害、1-MCP+乙烯、1-MCP+伤害处理的叶片中均不表达,由此可见CsWIP1仅受到乙烯信号传导途径调控.
|
| 关 键 词： | CsWIP1 伤害诱导基因 乙烯信号传导途径 qRealTime-PCR |
qReal Time-PCR Analysis of CsWIP1 Expression in Different Tissues of Citrus |
| |
| YANG Xue-lian,YAN Shu-tang,BEI Xue-jun,LIU Bin,WEI Zhao-xin.School of Horticulture , L,scape Architecture,Southwest University,Chongqing ,China,.Agriculture College of Guizhou University,Guiyang.qReal Time-PCR Analysis of CsWIP1 Expression in Different Tissues of Citrus[J].Journal of Southwest Agricultural University,2011,33(4). |
| |
| Authors: | YANG Xue-lian YAN Shu-tang BEI Xue-jun LIU Bin WEI Zhao-xinSchool of Horticulture L scape Architecture Southwest University Chongqing China Agriculture College of Guizhou University Guiyang |
| |
| Institution: | YANG Xue-lian1,2,YAN Shu-tang3,BEI Xue-jun1,LIU Bin1,WEI Zhao-xin11.School of Horticulture and Landscape Architecture,Southwest University,Chongqing 400716,China,2.Agriculture College of Guizhou University,Guiyang 550025,3.The Citrus Research Institute,Chinese Academy of Agricultural Sciences,Chongqing 400712 |
| |
| Abstract: | Wound-induced gene(CsWIP1) expression in citrus was detected with qReal Time-PCR in treatments of ethylene stress and mechanical wounding.The results showed that CsWIP1 was expressed only in leaf and fruit abscission zones(AZ-C) by exogenous ethylene treatment,but not expressed in the wounding treatment,1-MCP+ethylene treatment and 1-MCP+wounding treatment in leaves,thus suggesting that CsWIP1 expression is regulated through the ethylene signal transduction pathway only. |
| |
| Keywords: | CsWIP1 wound induced gene ethylene signal transduction pathway qRealTime-PCR |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《西南农业大学学报》浏览原始摘要信息 |
| 点击此处可从《西南农业大学学报》下载免费的PDF全文 |
|
