邓恩桉茎段组织培养试验初探

采集邓恩桉茎段进行组织培养。试验结果表明:半木质化或刚木质化的茎段用浓度为0.1%Hgcl2消毒6min,用无菌水冲洗3～4次,再用Hgcl2消毒2 min效果最好,杀伤率低,获得率高达67.5%;芽分化及增殖培养基用改良MS+3%糖+3%的琼脂+VC0.01 mg∕L+VB20.015 mg∕L+BA0.5 mg/L+NAA0.25 mg/L,增殖系数达4倍,芽生长正常,不产生"玻璃化"苗;在IBA0.5 mg/L+ABT0.25 mg/L及IBA0.5 mg/L+NAA0.5 mg/L两种激素组合对邓恩桉不定根诱导有一点作用,但效果不理想,生根率只有20%,愈伤头大,上杯不容易成活。

Primary study on tissue culture of stem segment of Eucalypts dunnii

Stem segments of Eucalypts dunnii were used for tissue culture in this paper.Best effect with low killing rate and up to 67.5% gain rate was obtained according to the following procedures.Firstly,semi-lignified or lignified stem segments were disinfected for 6 minutes with 0.1% Hgcl2,washed 3-4 times with sterile water,then disinfected with Hgcl2 for 2 minutes.Modified medium with MS +3% sugar +3% agar +VC 0.01 mg/L +VB20.015mg/L + BA0.5mg/L +NAA0.25mg/L was used for bud differentiation and proliferation.The multiplication coefficient was 4 times,and buds grew normally with no vitrification.The two hormone combination of 0.5mg/L IBA +0.25mg/L ABT and 0.5mg/L IBA +0.5mg/L NAA had some effects on root induction of Eucalypts dunnii.But the effects were not very good.The rooting rate was only 20% with big callus,and goblet cell didn't survive easily.