酿酒酵母WD-1发酵生产谷胱甘肽条件的研究

谷胱甘肽是一种重要的生物活性三肽,广泛应用于医药、食品添加剂和化妆品行业。试验在摇瓶水平上对酿酒酵母WD-1生物合成谷胱甘肽的条件进行研究,考察培养基组成、培养条件对酿酒酵母生物合成谷胱甘肽的影响,包括不同的碳源、氮源、无机盐、半胱氨酸、接种量和转速等。优化后的培养基组成为：葡萄糖2%,酵母粉4%,蛋白胨2%,NaCl 1%,MgSO₄·7H₂O 0.1%,KH₂PO₄ 0.5%,(NH₄)₂SO₄ 0.2%,Cys 2 mmol/L,在接种量5%,转速240 r/min,装液量30 mL的优化条件下谷胱甘肽的产量达223.22 mg/L,较初始条件提高了36.81%。

Fermentation process of glutathione production by Saccharomyces cerevisiae WD-1

Glutathione is an important kind of biologically active tripeptides. It is widely used as a pharmaceutical compound and has the potential to be used in food additives and cosmetic industry. The condition of glutathione biosynthesis by Saccharomyces cerevisiae WD-1 in shaking flasks was studied. The effect of medium compositions, including the selection and optimization of carbon source and organic nitrogen source, inorganic salt and cysteine on glutathione production was investigated. The optimal medium was determined as follows: glucose 2%, yeast extract 4%, peptone 2%, NaCl 1%, MgSO₄·7H₂O 0.1%, KH₂PO₄ 0.5%,(NH₄)₂SO₄ 0.2%, Cysteine 2 mmol·L^-1. Orthogonal experiment was carried out in order to determine the optimal conditions, which were as follows: the inoculum size 5%, agitation rate 240 r·min^-1 and the containing liquid 30 mL. In the optimal condition, the glutathione yield achieved 223.22 mg·L^-1,36.81% higher than the initial production．