| 蚯蚓细胞色素P450基因的克隆及序列分析 |
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| 引用本文： | 赵春,蒋美琳,周新初,鲁晓旭,艾晓杰,王秀红,邱江平,李银生.蚯蚓细胞色素P450基因的克隆及序列分析[J].上海交通大学学报(农业科学版),2015(4):33-37. |
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| 作者姓名： | 赵春 蒋美琳 周新初 鲁晓旭 艾晓杰 王秀红 邱江平 李银生 |
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| 作者单位： | 上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240,上海交通大学 农业与生物学院,上海 200240 |
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| 基金项目： | 国家自然科学基金(31172360) |
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| 摘 要： | 蚯蚓是一种土壤环境的指示生物,参与土壤中有毒化学物质的降解等过程。细胞色素P450酶是动物体内参与外源化合物代谢的主要酶系。本实验通过已有的其他物种的细胞色素P450酶保守序列设计简并引物,用RT-PCR的方法成功扩增出了赤子爱胜蚓体内的一条P450序列(670bp左右,Genbank登录号:KF823788),并对其保守区域、氨基酸构成及三级结构进行了分析。这是首次从蚯蚓体内扩增出P450基因,为下一步从基因层次研究蚯蚓机体对外源化合物的解毒功能及其机理提供了参考。
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| 关 键 词： | 蚯蚓 细胞色素P450酶 解毒机制 基因 氨基酸 三级结构 |
| 收稿时间： | 2014/10/19 0:00:00 |
Clone and Sequence Analysis of Cytochrome P450 Gene in Earthworm |
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| ZHAO Chun,JIANG Mei-lin,ZHOU Xin-chu,LU Xiao-xu,AI Xiao-jie,WANG Xiu-hong,QIU Jiang-ping and LI Yin-sheng.Clone and Sequence Analysis of Cytochrome P450 Gene in Earthworm[J].Journal of Shanghai Jiaotong University (Agricultural Science),2015(4):33-37. |
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| Authors: | ZHAO Chun JIANG Mei-lin ZHOU Xin-chu LU Xiao-xu AI Xiao-jie WANG Xiu-hong QIU Jiang-ping and LI Yin-sheng |
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| Institution: | School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China,School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China and School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China |
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| Abstract: | Earthworm is a kind of bio-indicators for soil environment.They participate in the degradation of toxic chemicals in the soil.Cytochrome P450 (CYP450) is an important super enzymatic family in organisms with the detoxification function of xenobiotics.In this study,the primers were designed based on the conserved sequence of reported CYP450 gene from other animal species.One pieces of CYP450 gene was cloned by RT-PCR and then sequenced.It was about 670 bp and the accession number in Genbank was KF823788.The conserved sequence,amino acid composition and its tertiary structure were then analyzed.This is the first report about CYP450 gene clone from earthworm.This study is of important sense for the further study about detoxification of xenobiotics and its mechanism in earthworm body in the gene level. |
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| Keywords: | earthworm cytochrome P450 detoxification sequence amino acid tertiary structure |
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