猪附红细胞体对树突状细胞成熟的影响研究

为了观察树突状细胞(dendritic cell，DC)2.4的表型和功能变化，以反映猪附红细胞体(Mycoplasma suis，M. suis)对DC成熟分化的影响。通过将DC2.4分成5组：空白对照组：不进行任何处理；实验组：M. suis 0.1、1.0、10.0 μL/mL组，分别在培养液中加入相应剂量的纯化M. suis；阳性对照组：加入脂多糖，终浓度1 μg/ml。应用流式细胞术检测DC2.4培养24 h后细胞表面抗原CD80、CD86、MHC-Ⅱ类分子的表达，并采用MTT法检测同基因混合淋巴细胞反应观察DC2.4对T细胞的抗原提呈能力。流式结果表明：M. suis刺激DC2.4后抑制表面分子CD80、CD86、MHC-Ⅱ的表达，表达量均低于阳性对照组(P<0.01)，且对DC2.4的抑制作用呈现剂量依赖。混合淋巴反应中M.suis显著抑制DC2.4对T细胞的激活能力，且阳性对照组与空白组之间差异有显著性意义(P<0.01)。本研究证明了M. suis可以抑制DC2.4的分化与成熟，M. suis刺激的DC2.4在免疫反应中发挥负性调节作用，为M. suis的免疫研究提供科研数据。

Effect study of Mycoplasma suis on the maturation of Dendritic Cells

To observe the phenotype and function changes of the dendritic cell (DC) 2.4 in the initial study of Mycoplasma suis (M. suis) on DC maturation. DC 2.4 was proliferated by cell culture. DC2.4 were divided into five groups: blank control group: there was no treatment; experimental group: M. suis 0.1, 1.0, 10 μL/mL groups, the purified M. suis with the corresponding dose were added in the culture medium; positive control group: lipopolysaccharide with final concentration of 1 μg/mL was added into the culture medium. There were 6 holes in each group. Expression of DC2.4 cell surface CD80, CD86, MHC-Ⅱ molecule in dendritic cells cultured for 24 hours were detected by flow cytometry, followed by parallel in vitro mixture with lymphocyte reaction. Types of DC on T cells with stimulation capacity were observed by MTT. The results of flow cytometry showed that, M. suis significantly inhibited DC2.4 surface molecules (CD80, CD86 and MHC-Ⅱ) expression (P<0.01), lower than which contracted with positive control group. In addition, M. suis significantly inhibited the dendritic cells to T cell activation capacity, and there were significant differences between positive control group and blank control group (P<0.01) in the mixed lymphocyte reaction. M. suis could inhibited the activation of dendritic cells mature, and the nagative regulatory role of the mature DC in immune function may provide research data for M. suis immunology study.