| 鹅源新城疫病毒F和HN基因的表达及功能鉴定 |
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| 引用本文： | 胡顺林,张艳梅,孙庆,吴艳涛,刘玉良,刘秀梵. 鹅源新城疫病毒F和HN基因的表达及功能鉴定[J]. 扬州大学学报(农业与生命科学版), 2006, 27(4): 8-11 |
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| 作者姓名： | 胡顺林 张艳梅 孙庆 吴艳涛 刘玉良 刘秀梵 |
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| 作者单位： | 扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009;扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009 |
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| 摘 要： | 鹅源新城疫病毒的F和HN基因经RT-PCR方法从尿囊液中扩增后分别克隆进pCR2．1载体,再分别亚克隆到真核表达载体pCI-neo上,构建了F基因的表达载体(pCI-F)和HN基因的表达载体(pCI-HN)。间接免疫荧光(IFA)鉴定结果表明:F和HN基因均能在细胞中得到较好的表达;将pCI-F和pCI-HN共转染CEF和COS细胞,转染的细胞中能形成明显的合胞体。而用pCI-F单独进行转染时,不能产生合胞体,说明F蛋白要发挥其融合作用必须要有HN蛋白的参与,同时也进一步证明F和HN蛋白与病毒的融合特性密切相关。
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| 关 键 词： | 鹅 新城疫病毒 F蛋白 表达 合胞体 |
| 文章编号： | 1671-4652（2006）04-0008-04 |
| 收稿时间： | 2006-05-24 |
| 修稿时间： | 2006-05-24 |
Expression and functional identification of F and HN gene from Newcastle disease virus of goose origin |
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| HU Shun-lin,ZHANG Hai-mei,SHUN Qing,WU Yan-tao,LIU Yu-liang,LIU Xiu-fan. Expression and functional identification of F and HN gene from Newcastle disease virus of goose origin[J]. Journal of Yangzhou University(Agricultural and Life Science Edition), 2006, 27(4): 8-11 |
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| Authors: | HU Shun-lin ZHANG Hai-mei SHUN Qing WU Yan-tao LIU Yu-liang LIU Xiu-fan |
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| Abstract: | F and HN gene of Newcastle disease virus of goose origin were amplified and cloned into pCR2.1 vector.F and HN gene from pCR2.1 vector were subcloned into eukaryotic expression vector,pCI-neo,to construct pCI-F and pCI- HN respectively.The positive clones were identified by enzyme digestion,PCR and sequencing.The results of IFA showed that F and HN gene expressed well in COS-1 cell.The experiment of cotransfection with pCI-F and pCI-HN revealed that co-expression of pCI-F and pCI-HN could induce syncytia formation on transfected cells,but the cells transfected with pCI- F alone couldn't form syncytia.The results mentioned above confirmed that F and HN protein were associated with the fu- sion function of NDV. |
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| Keywords: | goose Newcastle disease virus F protein expression syncytia |
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