一株绿磺隆降解菌的分离鉴定及其特性研究

从长期施用绿磺隆的土壤中采取土样 ,经驯化富集后筛选到一株能高效降解绿磺隆的细菌LHL 1菌株 ,经初步鉴定为黄单胞菌 (Xanthomonassp)。在此基础上 ,研究了该菌株对绿磺隆的降解特性。结果表明 :在绿磺隆浓度为 2 0mgL- 1的无机盐培养基中 ,3 0℃、1 5 0rmin- 1振摇 40h ,其对绿磺隆的降解率达 62 %。在所试的金属离子中 ,Pb2 、Ag 抑制其生长 ,Ba2 、Al3 、Zn2 等对其生长没有影响

ISOLATION,IDENTIFICATION AND CHARACTERS OF A CHLORSULFURON-DEGRADING STRAIN

Chlorsulfuron is a kind of herbicide that is used all over the world for the control of broad-leaved weeds in the field of cereal crops Increasing attention is being paid all over the world for its persistency and high toxicity Bioremediation is an effective and economic method to treat the environment that has been polluted by hazardous organic compounds So researchers paid much attention in this area;most of this research has been focused on the isolating and characterizing bacteria with abilities to degrade particular pollutants or transformation of metals. At the same time people have begun to explore genetic manipulation of genes encoding degradability of specific chemicals and applied genetic engineered microorganisms in the waste treatment of cleaning up Environmental biotechnology has become an indispensable component of our current society dealing with pollution reduction and cleaning up the contaminated sites A strain of Chlorsulfuron degrading bacteria was isolated from soil samples collected from field with frequent application of herbicide The strain was designated LHL-1 and preliminary identified as Xanthomonas sp About 62% of Chlorsulfuron was degraded when the strain was cultured in a mineral salt medium containing 20 mg L^-1 Chlorsulfuron under the condition of 30, 150 r min^-1, 40 h Among the tested metal ions, Pb²⁺ and Ag⁺ inhibited its growth, while Ba²⁺, Al³⁺, Zn²⁺ and other metal ions have no effect on its growth.