Towards development of Al-toxicity tolerant lines in indica rice by exploiting somaclonal variation |
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Authors: | Bidhan?Roy Email author" target="_blank">Asit?B?MandalEmail author |
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Institution: | (1) CARI, Biotechnology Section, Central Agricultural Research Institute, Port Blair, 744101, India |
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Abstract: | Somaclonal variations, induced in vitro, were used to enhance tolerance to aluminium (Al) toxicity in rice. Tolerant plants were developed through in vitro screening of embryogenic calli. The calli were derived from mature seed embryos and cultured on medium stressed with different
concentrations of Al2(SO4)3⋅18H2O. Seed germination, callus induction, plantlet regeneration and callus health declined with increased concentration of Al.
At higher Al concentrations, callus health deteriorated drastically with partial to total necrosis. Plantlet regeneration
varied largely among varieties and treatments. The variety IR72 produced maximum plantlets among all genotypes tested. An
amount of 60 ppm or more Al was highly toxic, which greatly reduced plantlet regeneration from callus. R0 plantlets were grown under glasshouse. Based on the appearance of bronzing symptoms on leaves, the tolerant R1 plants were selected. R1 and R2 lines derived from putative tolerant somaclones, were evaluated in fiberglass tanks filled with Al-toxic soil. R3 population was evaluated in the field. A few lines derived from IR72 showed high yield and good plant type. The progenies
at R3 showed normal root growth under stressed environment in sand culture. The study revealed that in vitro screening would be an appropriate alternative to conventional breeding in evolving Al-tolerant lines as observed in case
of other abiotic stresses. The technique was useful in creating de novo synthesized Al-tolerance character in rice. |
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Keywords: | aluminium-toxicity tolerance indica rice sand culture somaclonal variation |
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