Response of bovine and porcine peripheral blood mononuclear cells to human recombinant interleukin 2(125) |
| |
| Authors: | S Fong M V Doyle |
| |
| Affiliation: | 1. Department of Civil Engineering, School of Civil Engineering, Tianjin University, Tianjin 300072, China;2. Department of Geotechnical Engineering, College of Civil Engineering, Tongji University, Shanghai 200092, China;3. Departament of Civil and Environmental Engineering, Universitat Politècnica de Catalunya - BarcelonaTech, Jordi Girona 1-3, Barcelona 08034, Spain;4. Tongji Architectural Design (Group) Co., Ltd., No. 1230 Siping Road, Shanghai 200092, China;1. INENCO – Universidad Nacional de Salta – CONICET, Avda. Bolivia 5150, 4400 (Salta), Argentina;2. CONICET, CC302 Santa Rosa, 6300 (La Pampa) Argentina;1. Faculty of Civil Engineering, University of Guilan, Rasht, Iran;2. Faculty of Civil Engineering, Khajeh Nasir Toosi University of Technology, Tehran, Iran;1. University of Basel, Biogeography Research Group, Department of Environmental Sciences, Basel 4056, Switzerland;2. Tropical Biodiversity Section, Science Museo of Trento, Via della Scienza e del lavoro, 38122 Trento, Italy;3. Applied Biodiversity Research Division, South African National Biodiversity Institute, Claremont 7735, Cape Town, South Africa;4. Department of Botany and Zoology, Stellenbosch University, Matieland 7602, South Africa;5. Department of Life Sciences, The Natural History Museum, Cromwell Road, London SW7 5BD, UK;6. Ethiopian Wildlife Conservation Authority, P.O. Box 386, Addis Ababa, Ethiopia |
| |
| Abstract: | Bovine and porcine peripheral blood mononuclear cells (PBMC) were tested for their response to human recombinant interleukin 2(125) (rIL 2(125)). The rIL 2(125) used in these experiments was purified to homogeneity from Escherichia coli, contained a site-specific modification at amino acid #125 replacing a cysteine with a serine residue and had a specific activity of 4 X 10(6) units/mg. Human rIL 2(125) was shown to be directly mitogenic for bovine and porcine PBMC and was able to maintain the long-term growth of mitogen-activated PBMC of both species. Long-term cultures were highly sensitive to low levels of rIL 2(125) and showed dose-dependent responses when used in short-term IL 2 assays. Bovine and porcine PBMC preincubated with human rIL 2(125) for 1 and 5 days demonstrated enhanced levels of cell-mediated cytotoxicity against both allogeneic and xenogeneic cell lines. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
