基于SSR标记的菠萝种质DNA指纹图谱构建

以来自9个国家或地区的26份菠萝种质为材料，利用SSR标记进行了DNA指纹图谱的构建。筛选出多态性高、稳定性好的8对SSR引物共检测到35个多态性位点，每对引物的多态性位点数平均为4.38，引物的多态性条带百分比平均高达92.92％，PIC值介于0.26～0.39之间，平均为0.33。利用这8对SSR引物构建了26份菠萝种质的指纹图谱，并对菠萝种质做遗传相似性分析和聚类分析。结果表明，菠萝种质遗传相似系数分布在0.421～0.974之间，平均为0.689，26份菠萝种质在遗传相似系数0.633处被划分为3类。该研究结果将为菠萝种质鉴定、分类及分子育种提供重要科学依据。

Construction of DNA Fingerprint for Pineapple Germplasm Based on SSR Marker

DNA fingerprints were constructed for 26 pineapple lines originated from nine countries or regions based on SSR marker. Totally, 35 polymorphic alleles were detected using eight SSR primers with the higher polymorphism and the better stability, with an average 4.38 polymorphic alleles for each primer. For SSR primers, the percentages of polymorphic bands to total producing bands reached 92.92%, and the polymorphism information content values varied from 0.26 to 0.39 with an average of 0.33. DNA fingerprints were constructed for 26 pineapple germplasms with the eight SSR primers. The genetic similarity analysis and cluster analysis were also conducted for these pineapple lines, and the genetic similarities coefficient among pineapple lines ranged from 0.421 to 0.974 with an average of 0.689. The 26 pineapple germplasms were divided into three groups at the genetic similarities coefficient of 0.633. These results would provide an important scientific basis for pineapple germplasm identification, classification and molecular breeding of pineapple.