氟对牛脾淋巴细胞内抗氧化功能和NO代谢的影响

采用常规方法分离培养健康牛脾脏淋巴细胞，用不同浓度氟化钠（NaF）染毒24 h。检测牛脾淋巴细胞内谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）、一氧化氮合酶(NOS)活性及丙二醛（MDA）和一氧化氮（NO）的含量。结果表明，与对照组相比，淋巴细胞内GSH-Px、SOD活性不同程度的降低，而其余各指标均不同程度的升高，且存在着剂量效应关系。由此得出,氟能够导致体外培养牛脾淋巴细胞内抗氧化功能下降，脂质过氧化程度加重，自由基含量增多，对淋巴细胞造成损伤。

Effects of Fluorine on Antioxdative Activity and Metabolism of Nitric Oxide in the Splenic Lymphocyte of Cattle

Splenic lymphocyte isolated and cultured from cattle spleen was exposed to different of levels of sodium fluoride in 24 h.Detecting GSH-Px activity,SOD activity,NOS avtivity and contents of MDA and NO of splenic lymphocyte.The results showed that GSH-Px activity and SOD activity were higher and other indexes were lower in the lymphocyte exposed to fluorine than control ones and there was dose-effect relationship.Conclusion: it is demonstrated that fluorine could degrade antioxdative activity,aggravate the level of lipid peroxidation,increase the content of free radicals,and induce the damage of lymphocyte.