小麦族十一个物种的叶绿体psaC基因序列比较分析(简报)

植物叶绿体基因组的psaC基因有非常重要的功能,是植物光化学反应和光系统Ⅰ的组成成分。本研究对小麦族11个物种的psaC基因进行了扩增、测序和对比分析。结果表明,1)psaC基因编码区非常保守,存在3个SNP位点,但这3个突变位点都为同义突变,并没有引起氨基酸的改变。2)在psaC基因的间隔区,栽培大麦多了一个6 bp的‘CAAAAA’多核苷酸插入。在已经研究的植物中,该‘CAAAAA’多核苷酸插入是栽培大麦所特有的,表明该片段是在大麦物种分化以后插入的。该片段不但可以作为栽培大麦特异的标记序列,而且在大麦属物种的系统进化关系研究中有特殊的用途。3)发现的SNPs位点可用于相关物种的细胞质分子标记开发。

Sequence analysis of chloroplast psaC gene in eleven Triticeae species

The chloroplast psaC gene is an essential component,both for photochemical activity and stable assembly of photosystem I in higher plants.The psaC sequences from 11 Triticeae species were obtained and sequence analysis revealed that: 1) the psaC gene is highly conserved among species.Although there were three SNPs within the coding region of the gene,none of them resulted in changes in amino acid sequences;2) In the intergenic regions of the psaC gene,an additional insertion of six bases(CAAAAA) was only found in Hordeum vulgare thus this unique insertion can be used as a specific marker for H.vulgare;3) The SNPs can be used in molecular marker development.