| 犬瘟热病毒F基因克隆及序列分析 |
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| 引用本文： | 高华峰,朱建波,赵文华,姚俊,张念祖. 犬瘟热病毒F基因克隆及序列分析[J]. 动物医学进展, 2007, 28(7): 40-43 |
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| 作者姓名： | 高华峰 朱建波 赵文华 姚俊 张念祖 |
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| 作者单位： | 农业部热带亚热带动物病毒病重点实验室,云南昆明,650224;农业部热带亚热带动物病毒病重点实验室,云南昆明,650224;农业部热带亚热带动物病毒病重点实验室,云南昆明,650224;农业部热带亚热带动物病毒病重点实验室,云南昆明,650224;农业部热带亚热带动物病毒病重点实验室,云南昆明,650224 |
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| 摘 要： | 从犬瘟热病毒检测阳性的3份(KM1、KM2、KM3)病料中进行F基因的克隆测序,并与其他5株代表性参考毒株的同一基因序列进行比较(国内外流行毒株以及疫苗毒株).结果表明,KM1与KM2有95.7%的核苷酸同源性,其氨基酸序列完全相同;KM3株与KM1有1个氨基酸的差异,与参考的5株犬瘟热病毒的F基因核苷酸和氨基酸分析同源性分别为91.1%～96.5%和96.8%～100%.
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| 关 键 词： | 犬瘟热病毒 F基因 融合基因 序列分析 |
| 文章编号： | 1007-5038（2007）07-0040-04 |
| 修稿时间： | 2007-03-30 |
Cloning and Sequenceing of F Gene of Canine distemper virus |
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| GAO Hua-feng,ZHU Jian-bo,ZHAO When-hua,YAO Jun,ZHANG Nian-zu. Cloning and Sequenceing of F Gene of Canine distemper virus[J]. Progress In Veterinary Medicine, 2007, 28(7): 40-43 |
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| Authors: | GAO Hua-feng ZHU Jian-bo ZHAO When-hua YAO Jun ZHANG Nian-zu |
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| Affiliation: | The Key Laboratory of Tropical and Subtropical Animal Virology of Ministry of Agriculture, Kuunming, Yunan, 650224, China |
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| Abstract: | Suspicious canine distemper virus(CDV) samples collected from Kunming were amplified by using RT-PCR,in which 3 positive samples were detected.3 strains of canine distemper virus(named KM1,KM2,KM3) were used for protein F gene cloning and sequenceing and then compared with 5 representative reference strains.The results showed that the F gene from strain of KM1 and KM2 have the same amino acid sequence,but the strain of KM3 have a amino acid difference compared with the strain of KM1.At the nucleotide level KM1 shared the homologue of 91.1% to 96.5% and at the amino acid level,the homologue is from 96.8 % to 100% compared with related viruses. |
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| Keywords: | Canine distemper virus F gene fusion protein gene sequence analysis |
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