| 番鸭小肠碱性磷酸酶的分离纯化及部分性质研究 |
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| 引用本文： | 刘建昌,余万凯,池雪林,刘姗姗.番鸭小肠碱性磷酸酶的分离纯化及部分性质研究[J].福建农林大学学报(自然科学版),2010,39(2). |
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| 作者姓名： | 刘建昌 余万凯 池雪林 刘姗姗 |
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| 作者单位： | 福建农林大学动物科学学院,福建,福州,350002 |
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| 基金项目： | 福建省教育厅资助项目(JA08061) |
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| 摘 要： | 分离纯化番鸭小肠中碱性磷酸酶,并对其酶促动力学性质进行研究.结果表明:该酶催化磷酸苯二钠水解反应时最适温度为45℃,最适pH为9.72,米氏常数(Km)为4.76 mmol.L-1.Mg2+对碱性磷酸酶活性有明显激活作用;Cd2+浓度小于0.4 mg.L-1时对碱性磷酸酶有激活作用,而浓度高于0.4 mg.L-1时则有抑制作用;Cu2+对碱性磷酸酶活性有明显抑制作用.
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| 关 键 词： | 番鸭 碱性磷酸酶 酶活性 小肠 米氏常数 |
Isolation, purification and some properties of alkaline phosphatase from the small intestine of Muscovy |
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| LIU Jian-chang,YU Wan-kai,CHI Xue-lin,LIU Shan-shan.Isolation, purification and some properties of alkaline phosphatase from the small intestine of Muscovy[J].Journal of Fujian Agricultural and Forestry University,2010,39(2). |
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| Authors: | LIU Jian-chang YU Wan-kai CHI Xue-lin LIU Shan-shan |
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| Institution: | College of Animal Science;Fujian Agriculture and Forestry University;Fuzhou;Fujian 350002;China |
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| Abstract: | The alkaline phosphatase (AKP) was extracted and purified from the small intestine of Muscovy. The kinetic characters of AKP have been studied. The results showed that the optimum temperature was 45 ℃, and the optimum pH was 9.72 for hydrolysis of penylphosphoric acid disodium salt, the Michaelis constant (Km) was 4.76 mmol·L~(-1). Mg~(2+) can activate AKP significantly, Cd~(2+) can activate AKP at lower concentration than 0.4 mg·L~(-1), but inhibit AKP at higher concentration than 0.4 mg·L~(-1). Cu2+ inhibited AKP significantly. |
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| Keywords: | Muscovy duck alkaline phosphatase small intestine Michaelis constant |
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