条锈菌侵染慢锈性小麦品种川麦107后的蛋白质组学分析

为了在基因和蛋白表达水平上研究小麦的慢条锈性机制,运用双向电泳技术分析了条锈菌侵染的2个小麦品种(川麦107和80-8)的对照和发病14 d叶片的差异表达蛋白,从2-D图谱上找到9个差异表达蛋白,其中点P5是在接种并发病的川麦107中新诱导出的一个差异蛋白质.通过基质辅助激光解析电离飞行时间质谱技术(MALDI-TOF-MS)获得6个蛋白质的肽指纹图谱(PMF),数据库搜索鉴定出2个蛋白质.这2个蛋白质是磷酸核酮糖激酶(Phosphoribulokinase,PRK)和脱氢抗坏血酸还原酶(Dehydroascorbate reductase,DHAR).

Proteome Analysis of Slow-rusting Variety Chuanmai 107 Inoculated by Wheat Stripe Rust (Puccina striiformis)

The changes of proteins in two wheat varieties,Chuanmai107and 80-8,caused by the inoculation of Puccina striiformis f.sp.tritici were analyzed using 2-dimentional electrophoresitic technology.9 protein spots resolved on 2-D gels were found to be differentially expressed after analyzing Coomas-blue stained gel of the soluble protein samples from the control and disease-affected leaves at 14 days post inoculation.These spots were then excised from gel and analysed by matrix-assisted laser desorption ionisation time of flight mass spectrometry(MALDI-TOF-MS).Peptide mass fingerprinting (PMF) of 6 proteins were submitted to NCBI and Swiss-prot databases for protein identification.Protein spot P5 only induced in Chuanmai 107 inoculated by CY32 was an unknown protein.2 proteins identified were respectively phosphoribulokinase(PRK) and dehydroascorbate reductase(DHAR).Proteome analysis of slow-rusting variety,Chuanmai 107,may provide clues for elucidating the resistant mechanisms of slow-rusting variety.