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| 苏云金芽孢杆菌菌株B-Pr-88的生物学特性 | |
| 引用本文： | 李长友,张杰,宋福平,李国勋,黄大昉.苏云金芽孢杆菌菌株B-Pr-88的生物学特性[J].植物保护学报,2004,31(1):21-25. |
| 作者姓名： | 李长友 张杰 宋福平 李国勋 黄大昉 |
| 作者单位： | 1. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100094;山东莱阳农学院,莱阳,265200 2. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室,北京,100094 3. 山东莱阳农学院,莱阳,265200 4. 中国农业科学院生物技术研究所,北京,100081 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 就自行分离的、对鳞翅目夜蛾科害虫具有高毒力的Bt菌株B-Pr-88的生物学特性进行了系统研究.B-Pr-88可形成菱形、球形和不规则形等多种形态的晶体,主要由130kDa和60kDa两种蛋白组成;酯酶型与标准菌株蜡螟亚种和库斯塔克亚种一致,同属于galleriae型;10项生理生化反应结果表明,B-Pr-88与标准菌株蜡螟亚种反应一致;B-Pr-88菌株至少含有3种大小不同的质粒,经PCR-RFLP鉴定该菌株至少包括cry2Ab、cry9Ba和多种cry1(包括cry1Cb、cry1Db、cry1Fb和cry1Gb)等6种不同的杀虫晶体蛋白基因. |
| 关 键 词： | 苏云金芽孢杆菌 酯酶型 生理生化反应 杀虫晶体蛋白 cry基因 |
| 收稿时间： | 2003/4/11 0:00:00 |
| 修稿时间： | 2003年4月11日 |
Biological research on Bacillus thuringiensis strain B-Pr-88 |
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| Li Changyou,Zhang Jie,Song Fuping,Li Guoxun and Huang Dafang.Biological research on Bacillus thuringiensis strain B-Pr-88[J].Acta Phytophylacica Sinica,2004,31(1):21-25. | |
| Authors: | Li Changyou Zhang Jie Song Fuping Li Guoxun and Huang Dafang |
| Institution: | Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China;Laiyang Agricultural College, Laiyang 265200, China,Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China,Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China,Laiyang Agricultural College, Laiyang 265200, China and Institute of BiotechnologyResearch, Chinese Academy of Agricultural Sciences, Beijing 100081, China |
| Abstract: | The biological characteristics of the Bacillus thuringiensis strain B-Pr-88,highly toxic to the pests in Noctuidae(Lepidoptera),was studied.Crystal shape of B-Pr-88 was bipy-ramidal,spherical and irregular,130kDa and 60kDa ICPs were expressed in B-Pr-88.Results of esterase pattern identification showed that B-Pr-88 was the same as standard strains of B.t.galleriae and B.t.kurstaki.Biochemical reaction indicated that B-Pr-88 was identical with B.t.galleriae.Three plasmids were detected from B-Pr-88.Results of cry-type gene identification showed that B-Pr-88 contained cry1Cb,cry1Db,cry1Fb,cry1Gb,cry2Ab and cry9Bagenes. |
| Keywords: | Bacillus thuringiensis esterase biochemical reaction ICPs cry gene |
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