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苜蓿多糖提取中适宜品种及工艺的研究
引用本文:张东杰,黄明海,姜筱红,高玉荣,冯昆.苜蓿多糖提取中适宜品种及工艺的研究[J].黑龙江八一农垦大学学报,2003,15(3):93-96.
作者姓名:张东杰  黄明海  姜筱红  高玉荣  冯昆
作者单位:1. 黑龙江八一农垦大学食品学院,密山,158308
2. 黑龙江完达山乳业股份有限公司850分公司
3. 哈尔滨松花江啤酒厂
基金项目:黑龙江省农垦总局科技攻关项目(HKK95-10-01-02)
摘    要:苜蓿含有具有生物活性的食用多糖,本实验对在黑龙江省主栽和试栽的国内外16个苜蓿品种的多糖含量进行了测定比较.对其提取工艺进行研究,利用热水浸提、醇沉等方法可得到粗多糖,采用木瓜蛋白酶和三氯乙酸进一步处理,结果表明浸提3次、木瓜蛋白酶添加0.05%、三氯乙酸终浓度达到6%为宜。

关 键 词:苜蓿  多糖  木瓜蛋白酶  三氯乙酸  提取工艺
文章编号:1002-2090(2003)03-0093-04
修稿时间:2003年4月10日

The Study on Varieties and The Technology of Extracting Clover Polysaccharides
ZHANG Dong-jie,HUANG Ming-hai,JIANG Xiao-hong et al..The Study on Varieties and The Technology of Extracting Clover Polysaccharides[J].Journal of Heilongjiang August First Land Reclamation University,2003,15(3):93-96.
Authors:ZHANG Dong-jie  HUANG Ming-hai  JIANG Xiao-hong
Abstract:There are plenty of biologically active edible polysacchrides in Medicago Sativa L.In the experiment the contents of polysacchrides of sixteen internal and external kinds of Medicago Sativa L were detected and compared which were mainly and experimentally planted in Heilongjiang province. The extracting technology was studied. After Medicago Sativa L were diffused by hot water and precipitated by ethanol and processed by papain and trichlorocetic acid, the crude polysacchrides were get. The result showed diffuse three times and 0.05 percent dose of papain and ultimate content of trichlorocetic acid were optimal.
Keywords:Clover  polysaccharides  papain  trichlorocetic acid  extracting technology  
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