| 金黄色葡萄球菌诱发建立乳房炎小鼠病理模型 |
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| 引用本文： | 谭炳海,张桂林,曹荣峰. 金黄色葡萄球菌诱发建立乳房炎小鼠病理模型[J]. 中国畜牧兽医, 2010, 37(3): 158-159 |
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| 作者姓名： | 谭炳海 张桂林 曹荣峰 |
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| 作者单位： | （青岛农业大学动物科技学院，青岛 266109） |
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| 摘 要： | 为了建立小鼠乳房炎模型,将30只昆明小鼠随机分成3组,分别经第4对乳腺注入50μL的生理盐水、1.0×105CFU/mL和2.0×105CFU/mL的金黄色葡萄球菌,观察乳腺组织的外形和组织切片的变化;结果表明,注菌后24 h,小鼠出现明显的临床症状,乳腺出现不同程度的炎性症状,组织病理学显示腺泡腔内有大量嗜中性粒细胞浸润,泡腔间隔增宽。说明金黄色葡萄球菌能够诱发试验性乳房炎。
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| 关 键 词： | 小鼠 金黄色葡萄球菌 乳房炎 病理模型 |
The Establishment of Mouse Mastitis Patho-model Induced by Staphylococcus aureus |
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| TAN Bing-hai,ZHANG Gui-lin,CAO Rong-feng. The Establishment of Mouse Mastitis Patho-model Induced by Staphylococcus aureus[J]. China Animal Husbandry & Veterinary Medicine, 2010, 37(3): 158-159 |
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| Authors: | TAN Bing-hai ZHANG Gui-lin CAO Rong-feng |
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| Affiliation: | (College of Animal Science and Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China) |
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| Abstract: | 30 Kunming mice were divided into 3 groups random to establish the mouse mastitis patho-model. 30 mice were injected separately with 50 μL sterile physiological saline，1.0×105 CFU/mL and 2.0×105 CFU/mL Staphylococcus aureus in the forth lacteal gland. The appearance of lacteal gland and the diversity of histological section were observed. The results indicated that the mice 24 h after being injected with bacterium appeared evident clinical symptoms; the different degree inflammation was found in the lacteal gland; plenty of neutrophilic granulocyte infiltrated in the acinar lumina and the compartment became widen were discovered by the method of histopathology. The result indicated that the mouse mastitis model was established by the Staphylococcus aureus. |
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| Keywords: | mouse Staphylococcus aureus mastitis patho-model |
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