枸杞枝叶中乳酸菌的分离及其在徒长枝青贮中的应用评价

枸杞徒长枝是枸杞种植过程中的废弃物,但富含与枸杞子类似的营养成分和药用成分,将其开发为青贮饲料可以变废为宝.乳酸菌是青贮的关键菌种,从青贮原料上筛选的乳酸菌具有更好的适应性.采用MRS+CaCO3平板从枸杞枝叶上分离乳酸菌,筛选生长快、产酸能力强的菌株,作为添加菌剂应用于枸杞徒长枝青贮,评价青贮品质及测定有效成分含量,...

Isolation of Lactic Acid Bacteria from Branches and Leaves of Goji and Evaluation of Its Application in Epicormic Silage#br#

Epicormic of Goji are wastes from the planting process of Goji, and they are rich in nutrients and medicinal ingredients similar to the Gojizi, so they can be used as silage to turn waste into treasure. Lactic acid bacteria are the key species of silage, and those screened from silage raw materials have better adaptability. The MRS+CaCO₃ plate was used to isolate lactic acid bacteria from the branches and leaves of Goji epicormic, and gained the strains with fast-growing and strong acid-producing ability, which was used as an additive in the silage of Goji epicormic to evaluate the quality of silage and to screen of excellent lactic acid bacteria suitable for silaging of Goji epicormic. The results showed that 15 strains of lactic acid bacteria with large dissolving circles were isolated from the branches and leaves of Goji epicormic. Among them, GJ2, GJ8, and GJ18 grew faster and had strong acid production capacity. After adding strain GJ8 as a bacterial agent to silage Goji epicormic of branches, the crude protein, crude ash, acid detergent fiber, neutral detergent fiber, soluble carbohydrate, ammonia nitrogen, lactic acid, dry matter and other nutrients, as well as wolfberry polysaccharide, betaine, flavonoids and other effective ingredients content and sensory evaluation were the best. The 16S rDNA sequence analysis showed that the GJ8 strain was Pediococcus acidilactici. GJ8 could be used as a candidate strain for the development of special microbial agents for Goji silage.