旱盐交叉胁迫对甜高粱种子萌发和生理特性的影响

为探讨干旱与盐胁迫及其交互作用对甜高粱种子萌发及早期幼苗生长及生理特性的影响,以辽甜1号甜高粱为试验材料,采用PEG-6000(0、50、100g·L-1)和NaC1(0、75、150mmol·L-1)模拟不同程度的干旱胁迫和盐胁迫,研究单一胁迫及交互胁迫对甜高粱种子萌发特征和早期幼苗生理特性的影响.结果表明:单一胁迫...

Effects of Salt and Drought Cross Stress on Germination and Physiological Characteristics of Sweet Sorghum Seeds

In order to study effects of cross stress induced by salt and drought on seed germination and physiological characteristics of early seedlings of sweet sorghum，Liaotian No.1 sweet sorghum seed was used as the test material. PEG-6000 (0, 50 and 100 g·L^-1) was used to simulate drought stress, and NaCl (0, 75 and 150 mmol·L^-1) was used to simulate salt stress. The effects of drought and salt stress on the germination characteristics of sweet sorghum seeds and physiological characteristics of early seedlings were studied. The results showed that: single or cross stress could inhibit the germination and seedling transformation of sweet sorghum seeds. With the deepening of stress, the antioxidant activity, soluble protein and proline (Pro) content of the seedlings showed an increasing and then decreasing trend, and the malondialdehyde (MDA) and glutathione (GSH) content increased gradually. Compared with single stress, interactive stress under specific conditions could promote the germination of sweet sorghum seeds, enhance the antioxidant activity of seedlings, and increase the content of soluble protein, Pro, MDA and GSH. The results of two-factor variance analysis showed that the effects of single stress on seed germination and seedling physiology of sweet sorghum were more greater than that of drought salt cross stress. According to the comprehensive evaluation of the membership function, sweet sorghum of the stress tested was found as follows: interaction stress> single PEG stress> single NaCl stress. The effects of drought salt cross stress on seed germination and physiological characteristics of sweet sorghum seedlings were not simply superimposed by single stress. Compared with single stress, drought salt cross stress could alleviate the effects of salt stress on seed germination and seedling growth physiology of sweet sorghum to a certain extent.