侵染木薯的两种单组分菜豆金色花叶病毒属病毒及卫星分子基因组结构特征分析

为明确木薯(Manihot esculenta Crantz)植株叶片皱缩、畸形是否由菜豆金色花叶病毒属病毒侵染引起,从云南省红河州田间采集具有疑似感染症状的木薯植株叶片样品,应用菜豆金色花叶病毒属病毒简并引物、种专化性引物及卫星分子引物进行PCR扩增、克隆,通过测序分析其核苷酸序列特征并对其进行系统进化分析.结果 显...

Molecular characterization of two monopartite begomoviruses associated satellites from Manihot esculenta Crantz

In order to determine whether the crumpling symptoms of Manihot esculenta Crantz were caused by the infection of begomoviruses. Leaf samples of M. esculenta plants with suspected viral symptoms were collec-ted from the fields in Honghe, Yunnan. Four complete DNA-A and six betasatellite genome sequences were obtained by PCR, cloned, and sequenced. Comparisons performed with the complete nucleotide sequence revealed the presence of two begomoviruses, tobacco curly shoot virus (TbCSV) and ageratum yellow vein China virus (AYVCNV) in the M. esculenta samples. The TbCSV isolate from M. esculenta was most closely related to TbCSV-YN2247 (KX290925) isolated from Yunnan (with 96.67% sequence identity). The AYVCNV isolates from M. esculenta were most closely related to AYVCNV-YN4326 (KU601622) (with 95.80% sequence identity). Two betasatellites, malvastrum yellow vein betasatellite (MaYVB) and ageratum yellow vein China virus betasatellite (AYVCNVB) were detected in the M. esculenta samples. The MaYVB-YN6332-12 isolates were most closely related to MaYVB-Y216 (KX290925) (with 96.4% sequence identity) , whereas, the AYVCNB-YN6338-17 isolates were most closely related to AYVCNB-Hn9 (KU601622) (with 90.4% sequence identity). This study represents M. esculenta plants as a new host of TbCSV and AYVCNV, also the first report of mono-partite begomoviruses and associated DNA betasatellites complex infecting M. esculenta.