1株分离自云南省师宗县库蠓的新型西藏环状病毒

西藏环状病毒(Tibet orbivirus,TIBOV)为环状病毒属的新成员,病毒于2009年首次从我国西藏墨脱县采集的圆斑按蚊中分离,目前,对该病毒的认识仍十分有限.本研究对1株分离自我国云南省师宗县库蠓的新型TIBOV毒株YNSZ/V290/2019进行了鉴定,结果表明,病毒可在C6/36与BHK-21细胞上引起...

Isolation and Identification of a Novel Type Tibet Orbivirus Strain from Culicoides Collected in Shizong County of Yunnan Province

Tibet orbivirus (TIBOV) is a new member of the genus Orbivirus. The virus was first isolated from Anopheles maculatus collected in Motuo County, Tibet, China in 2009. At present, the recognition of this virus is still very limited. This is the first report on the isolation and identification of a new TIBOV strain YNSZ/V290/2019 from Culicoides collected in Shizong County, Yunnan Province, China. YNSZ/V290/2019 can cause an obvious cytopathic effect on C6/36 and BHK-21 cells. Under the electron microscope, the virus particles are spherical, with a diameter of about 55-75 nm, and have typical morphological characteristics of cycloviruses. The results of agarose gel electrophoresis showed that the genome of the virus was a 10 segment double-stranded RNA, showing the characteristics of "3-3-3-1". The sequence analysis of the highly conserved gene (Seg-3) and proteins (T2) showed that YNSZ/V290/2019 was a member of TIBOV, and YNSZ/V290/2019 shared 80.90%-81.70% nucleic acid sequence identity and 97.20%-97.30% amino acid sequence identity with other TIBOV strains. The sequence analysis of the high variable gene (Seg-2) and proteins (OC1) which determine the serotype of the Orbivirus showed that YNSZ/V290/2019 shared 26.90%-30.60% nucleic acid sequence identity and 28.5%-33.2% amino acid sequence identity with the known TIBOV strains, suggested that the strain may represent a new serotype of TIBOV. Serum neutralization tests were carried out on 40 cattle and 68 goat serum samples collected from Shizong pasture. The results showed that the positive rates of new TIBOV of cattle and sheep were 22.50% and 4.41%, respectively. The discovery of a new type of TIOBV in China enriches the research on arbovirus in China and provides a basis for further study on the distribution, pathogenicity, and host range of infection and development of diagnostic reagents of TIBOV in China.