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| 小麦纹枯病菌Rc-RPA-LFD快速检测方法的建立及应用 | |
| 引用本文： | 鞠玉亮,沈鹏飞,冯艳娟,羊国根,刘文成,郭文华,潘月敏. 小麦纹枯病菌Rc-RPA-LFD快速检测方法的建立及应用[J]. 植物病理学报, 2021, 50(5): 618-621 |
| 作者姓名： | 鞠玉亮 沈鹏飞 冯艳娟 羊国根 刘文成 郭文华 潘月敏 |
| 作者单位： | 安徽农业大学植物保护学院,植物病虫害生物学与绿色防控安徽普通高校重点实验室,安徽省绿色农药研发与应用创新团队,合肥 230036; 宿州市埇桥区农业技术推广中心,宿州 234000 |
| 基金项目： | 国家重点研发计划子课题(2017YFD2021708);安徽省科技重大专项(17030701050) |
| 摘 要： | |
Development and application of Rc-RPA-LFD for the rapid detection of Rhizoctonia cerealis |
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| JU Yu-liang,SHEN Peng-fei,FENG Yan-juan,YANG Guo-gen,LIU Wen-cheng,GUO Wen-hua,PAN Yue-min. Development and application of Rc-RPA-LFD for the rapid detection of Rhizoctonia cerealis[J]. Acta Phytopathologica Sinica, 2021, 50(5): 618-621 | |
| Authors: | JU Yu-liang SHEN Peng-fei FENG Yan-juan YANG Guo-gen LIU Wen-cheng GUO Wen-hua PAN Yue-min |
| Affiliation: | Key Laboratory of Biology and Sustainable Management of Plant Disease and Pests of Anhui Higher Education Institutes, Innovation Team of Green Pesticide Development and Application in Anhui Province, Anhui Agricultural University, Hefei 230036, China; Agricultural Technology Promotion Center of Yongqiao District, Suzhou 234000, China |
| Abstract: | Rhizoctonia cerealis is a soil-borne phytopathogenic fungus that causes wheat sharp eyespot, resulting in serious economic losses. In this study, the recombinase polymerase amplification combined with lateral-flow dipstick technology (Rc-RPA-LFD) was developed for the rapid and sensitivity detection of R. cerealis. The Rc-RPA-LFD assay could be completed at isothermal temperature of 38°C within 30 min without PCR thermal cyclers. The RPA primers and probe designed based on ITS region, showed high specificity to R. cerealis. The detection limit of Rc-RPA-LFD assay was 1 pg·μL-1 fungal genomic DNA, showed an equal sensitivity to that of conventional PCR. In addition, the Rc-RPA-LFD assay could detect R. cerealis from field soil samples, showing no significant differences compared to conventional PCR assay. The simplicity, rapidly and practicability all indicated that Rc-RPA-LFD assay will be a promising molecular diagnosis for the accurate and rapid detection of R. cerealis. |
| Keywords: | wheat sharp eyespot recombinase polymerase amplification specifically sensitivity practicality |
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