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| 中国小麦花叶病毒弱毒突变体筛选与鉴定 | |
| 引用本文： | 许小洁,迟云化,于伟鹏,游朋朋,何梦君,耿超,田延平,李向东. 中国小麦花叶病毒弱毒突变体筛选与鉴定[J]. 植物病理学报, 2021, 51(2): 217-224 |
| 作者姓名： | 许小洁 迟云化 于伟鹏 游朋朋 何梦君 耿超 田延平 李向东 |
| 作者单位： | 山东农业大学植物保护学院植物病理学系,泰安 271018; 山东省农业微生物重点实验室,泰安 271018 |
| 基金项目： | 农业公益性行业科技项目(201303021);山东省现代农业产业技术体系(SDAIT-02-10);泰山学者建设工程(ts201712023) |
| 摘 要： | 中国小麦花叶病毒(Chinese wheat mosaic virus,CWMV)是我国小麦土传花叶病毒病的重要病原.将CWMV RNA1、RNA2片段分别连接到pCB301-Rz载体,得到重组质粒pCWMV-RNA1和pCWMV-RNA2,这2个质粒可在本氏烟上引起花叶和畸形症状,在小麦上引起黄花叶症状.在pCWMV... |
| 关 键 词： | 中国小麦花叶病毒 侵染性cDNA克隆 致病力 衣壳蛋白 复制酶 |
| 收稿时间： | 2019-06-02 |
Screening and identification of attenuated mutants of Chinese wheat mosaic virus |
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| XU Xiao-jie,CHI Yun-hua,YU Wei-peng,YOU Peng-peng,HE Meng-jun,GENG Chao,TIAN Yan-ping,LI Xiang-dong. Screening and identification of attenuated mutants of Chinese wheat mosaic virus[J]. Acta Phytopathologica Sinica, 2021, 51(2): 217-224 | |
| Authors: | XU Xiao-jie CHI Yun-hua YU Wei-peng YOU Peng-peng HE Meng-jun GENG Chao TIAN Yan-ping LI Xiang-dong |
| Affiliation: | Department of Plant Pathology, College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China; Shandong Provincial Key Lab of Agricultural Microbiology, Tai'an 271018, China |
| Abstract: | Chinese wheat mosaic virus (CWMV) is one of the most economically important virus causing wheat soil-borne mosaic virus disease in China. The RNA 1 and RNA 2 fragments of CWMV were ligated to pCB301-Rz vector, producing recombinant plasmids pCWMV-RNA1 and pCWMV-RNA2, which could induce mosaic and deformation on Nicotiana benthamiana plants and yellow mosaic on wheat leaves. The genes for green fluorescent protein (GFP) was cloned to the 5'- end of the cysteine-rich gene of pCWMV-RNA2 and could produce green fluorescence in N. benthamiana plants. The codons for Asp (D1001) of RdRp in RNA1 and Glu (E127) of CP in RNA2 were mutated to that for Ala. Compared with wild-type CWMV, the resulting mutants, CWMV-RdRp-D1001A, CWMV-CP-E127A and CWMV-RdRp-D1001A/CP-E127A, showed significantly lower virulence and less CP accumulation in N. benthamiana plants. |
| Keywords: | Chinese wheat mosaic virus infectious cDNA clone virulence coat protein RNA-dependent-RNA polymerase |
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